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71188 RoboPop™ Ni-NTA His•Bind® Purification Kit

High-throughput, milligram-scale purification of His•Tag® fusion proteins

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      The RoboPop™ Ni-NTA His•Bind® Purification Kit is designed for filtration-based 96-well format purification of soluble His•Tag® fusion proteins directly from E. coli cultures without harvesting cells. The kit features PopCulture® Reagent, rLysozyme™ Solution, and Benzonase® Nuclease for centrifuge-free cell lysis and extract preparation in one step. The combination of PopCulture extraction, Ni-NTA His•Bind Resin, and a 2-ml filter plate allows high-throughput processing of up to 5 ml of E. coli culture per well. Whereas the magnetic-based His•Mag™ kit purifies up to 125 µg target protein per 1 ml culture, the filtration-based kit purifies up to 1 mg His•Tag fusion protein per 5 ml culture.

      Bacterial culture, cell lysis, and resin binding steps are carried out in standard 24- well plates (not supplied), which accommodate a maximum volume of 5 ml per well. The reaction slurry is then transferred to a 96-well Filter Plate (included) and the washing and elution steps are carried out on a vacuum manifold. The Filter Plate is compatible with standard filter manifolds for manual sample processing, and the entire purification has been validated for robotic sample processing with the Genesis® Freedom™ Workstation from Tecan and the MultiPROBE® II liquid handling work station from PerkinElmer Life Sciences. A 96-well Collection Plate (1-ml wells) with an air-tight aluminum foil sealer is provided for storage of the purified proteins.

      Description

      Catalogue Number71188 Brand Family Novagen®
      Features and benefits
      • High-throughput, 96-well protein purification directly from E. coli without harvesting cells
      • Centrifuge-free cell lysis and extract preparation in one step
      • Large-scale culture processing—up to 5 ml per well
      • High protein yield—up to 1 mg per well
      • Validated for robotic sample processing with the Genesis® Freedom™ Workstation from Tecan and the Packard-brand MultiPROBE® II liquid handling work station from PerkinElmer Life Sciences
      References
      ReferencesGrabski, A., et al. 2002. inNovations 14, 2.
      Product Information
      •75 mlPopCulture® Reagent
      •25 mlNi-NTA His•Bind Resin
      •125 ml4X Ni-NTA Bind Buffer
      •2 x 125 ml4X Ni-NTA Wash Buffer
      •50 ml4X Ni-NTA Elute Buffer
      •12-ml 96-well Filter Plate
      •11-ml 96-well Collection Plate
      with Sealer
      •300 KUrLysozyme™ Solution
      (Note: 1 KU = 1000 units)
      •1 mlrLysozyme Dilution Buffer
      •10,000 UBenzonase® Nuclease,
      Purity > 90%
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
      Storage Multiple storage requirements
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      71188 0