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HTS019RTA Ready-to-Assay™ CB1 Cannabinoid Receptor Frozen Cells

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Host Cell: Chem-1 Entrez Gene Number: X54937
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HTS019RTA
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      Tableau de caractéristiques principal

      SpeciesHost CellsProtein Target
      Human Chem-1CB1
      Description
      Catalogue NumberHTS019RTA
      Trade Name
      • Ready-to-Assay
      DescriptionReady-to-Assay™ CB1 Cannabinoid Receptor Frozen Cells
      Background InformationEMD Millipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. EMD Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

      CB1 is a GPCR that is expressed primarily in brain and nervous tissue, and mediates numerous CNS responses such as analgesia, appetite, cognition, memory and locomotor activity. A number of cannabinoid ligands bind to CB1 and activate Gi/o-mediated downstream responses, including inhibition of cAMP production and activation of ion channels and MAP kinases. Such ligands include exogenous agonists such as 9-THC, the main psychoactive component of the plant Cannabis sativa, and endogenous agonists such as anandamide that belong to eicosanoid family. A number of synthetic agonists such as CP55940 and R-(+)-WIN55212, and antagonists, such as SR141716A, for CB1 have been developed (Howlett et al., 2002). CB1 agonists have clinical utility in analgesia and antiemetic properties, whereas CB1 antagonists show promise for treatment of appetite in obesity disorders. EMD Millipore’s cloned human CB1-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant CB1 expression on the cell surface and contains high levels of the promiscuous G protein G15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists and antagonists of CB1.
      References
      Product Information
      Components
      • Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
      • Fifty (50) mL of Media Component.
      Detection methodFluorescent
      Quality LevelMQ100
      Applications
      Key Applications
      • Calcium Flux Assays
      • cAMP Assays
      Biological Information
      Entrez Gene Number
      GPCR ClassA
      Host CellsChem-1
      Protein TargetCB1
      Target Sub-FamilyCannabinoid
      Cell Line Type
      • GPCR Cell Lines
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size1 kit
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      HTS019RTA 04053252630002

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      Catégories

      Life Science Research > Cell Culture and Systems > Cell Lines > GPCR Frozen Cells
      Life Science Research > Drug Discovery and Development > GPCR Cell Lines & Assays > GPCR Frozen Cells