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411550 Mouse Anti-Human IgG, Fc Fragment Specific (HP6043) Peroxidase Conjugate

This Mouse Anti-Human IgG, Fc Fragment Specific (HP6043) Peroxidase Conjugate is validated for use in Enzyme Immunoassay for the detection of Human IgG, Fc Fragment Specific.

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411550
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411550-500UG
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      Description
      Catalogue Number411550
      Brand Family Calbiochem®
      References
      ReferencesHamilton, R.G. 1993. Clin. Chem. 39, 1988.
      Hamilton, R.G. and Morrison, S.M. 1993. J. Immunolo. Meth. 158, 107.
      Hamilton, R.G. 1990. Ann. Biol. Clin. 48, 473.
      Hamilton, R.G., et al. 1988. J. Immunoassay. 9, 275.
      Hamilton, R.G., et al. 1987. Hybridoma 6, 204.
      Nakane, P.K. and Kawaoi A., 1974. J. Histochem Cytochem. 22, 1084.
      Product Information
      FormLiquid
      FormulationIn PBS, 10 mg/ml BSA.
      PreservativeNone
      Quality LevelMQ200
      Applications
      Application ReferencesEnzyme Immunoassay Hamilton, R.G., et al. 1987. Hybridoma 6, 204. Cross-Reactivity, Myeloma Proteins Hamilton, R.G., et al. 1988. J. Immunoassay. 9, 275. Hamilton, R.G., et al. 1987. Hybridoma 6, 204.
      Key Applications Enzyme Immunoassay
      Application NotesEnzyme Immunoassay (1:1000, see application references)
      Application CommentsAntibody should be titrated for optimal results in individual systems.

      Cross-reactivity by ELISA against human myeloma proteins:
      Human IgG1: 100%
      Human IgG2: 100%
      Human IgG3: 100%
      Human IgG4: 100%
      Human IgM: <0.2%
      Human IgA: <0.1%
      Human IgE: <0.1%

      Cross-reactivity by ELISA against chimeric antibodies:
      Human IgG1: 100%
      Human IgG2: 100%
      Human IgG3: 100%
      Human IgG4: 100%
      Human IgM: <0.01%
      Human IgA: <0.01%
      Human IgE: <0.01%

      There is no detectable binding to bovine, goat, horse sheep IgG, so these species sera can be used for assay blocking buffers. Modification of Nakane and Kawaoi method used for enzyme coupling.
      Biological Information
      CloneHP6043
      HostMouse
      IsotypeIgG
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). Avoid more than 5 freeze/thaw cycles.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      411550-500UG 07790788060275

      Documentation

      Mouse Anti-Human IgG, Fc Fragment Specific (HP6043) Peroxidase Conjugate FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Mouse Anti-Human IgG, Fc Fragment Specific (HP6043) Peroxidase Conjugate Certificats d'analyse

      TitreNuméro de lot
      411550

      Références bibliographiques

      Aperçu de la référence bibliographique
      Hamilton, R.G. 1993. Clin. Chem. 39, 1988.
      Hamilton, R.G. and Morrison, S.M. 1993. J. Immunolo. Meth. 158, 107.
      Hamilton, R.G. 1990. Ann. Biol. Clin. 48, 473.
      Hamilton, R.G., et al. 1988. J. Immunoassay. 9, 275.
      Hamilton, R.G., et al. 1987. Hybridoma 6, 204.
      Nakane, P.K. and Kawaoi A., 1974. J. Histochem Cytochem. 22, 1084.
      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision11-March-2009 JSW
      ApplicationEnzyme Immunoassay (1:1000, see application references)
      DescriptionPurified mouse monoclonal antibody conjugated to horseradish peroxidase. Recognizes human IgG Fc fragment.
      BackgroundThe HP series of murine human Ig-specific monoclonal antibodies was produced from documented hybridoma cell lines that were developed at the Centers for Disease Control in Atlanta, Georgia. Laboratory analysis of each monoclonal antibody lot is performed in two stages during the production of ascites. First, the cell culture medium is analyzed for the presence and relative amount of human Ig-specific antibody by ELISA before injection of cells into mice. Second, collected ascites are analyzed for potency and antibody specificity using ELISA and isoelectric focusing (IEF), often in combination with an affinity immunoblot. For more information, the CALBIOCHEM® technical monograph, The Human IgG Subclasses, is available free of charge.
      HostMouse
      CloneHP6043
      IsotypeIgG
      FormLiquid
      FormulationIn PBS, 10 mg/ml BSA.
      Concentration Label Please refer to vial label for lot-specific concentration
      PreservativeNone
      CommentsAntibody should be titrated for optimal results in individual systems.

      Cross-reactivity by ELISA against human myeloma proteins:
      Human IgG1: 100%
      Human IgG2: 100%
      Human IgG3: 100%
      Human IgG4: 100%
      Human IgM: <0.2%
      Human IgA: <0.1%
      Human IgE: <0.1%

      Cross-reactivity by ELISA against chimeric antibodies:
      Human IgG1: 100%
      Human IgG2: 100%
      Human IgG3: 100%
      Human IgG4: 100%
      Human IgM: <0.01%
      Human IgA: <0.01%
      Human IgE: <0.01%

      There is no detectable binding to bovine, goat, horse sheep IgG, so these species sera can be used for assay blocking buffers. Modification of Nakane and Kawaoi method used for enzyme coupling.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). Avoid more than 5 freeze/thaw cycles.
      Toxicity Standard Handling
      ReferencesHamilton, R.G. 1993. Clin. Chem. 39, 1988.
      Hamilton, R.G. and Morrison, S.M. 1993. J. Immunolo. Meth. 158, 107.
      Hamilton, R.G. 1990. Ann. Biol. Clin. 48, 473.
      Hamilton, R.G., et al. 1988. J. Immunoassay. 9, 275.
      Hamilton, R.G., et al. 1987. Hybridoma 6, 204.
      Nakane, P.K. and Kawaoi A., 1974. J. Histochem Cytochem. 22, 1084.
      Application referencesEnzyme Immunoassay Hamilton, R.G., et al. 1987. Hybridoma 6, 204. Cross-Reactivity, Myeloma Proteins Hamilton, R.G., et al. 1988. J. Immunoassay. 9, 275. Hamilton, R.G., et al. 1987. Hybridoma 6, 204.