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12-421 MYPT1, (714-1004)

MYPT1, (714-1004) : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
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12-421
50 µg  
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      Replacement Information
      Description
      Catalogue Number12-421
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionMYPT1, (714-1004)
      References
      Product Information
      Presentation50mM Tris-HCl pH 7.5, 150mM NaCl, 0.1mM EGTA, 0.1% 2-mercaptoethanol, 0.03% Brij-35, 1mM benzamidine, 0.2mM PMSF and 50% glycerol
      Quality LevelMQ100
      Applications
      Key Applications
      • Kinase Assay
      Biological Information
      Entrez Gene Number
      Entrez Gene SummaryMyosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase.
      Gene Symbol
      • PPP1R12A
      • MYPT1
      • MGC133042
      • MBS
      Protein TargetROKα/ROCK-II
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O14974 # Regulates myosin phosphatase activity.
      SIZE: 1030 amino acids; 115281 Da
      SUBUNIT: PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, and one or several targeting or regulatory subunits. PPP1R12A mediates binding to myosin. Interacts with ARHA and CIT (By similarity). Binds PPP1R12B, ROCK1 and IL16.
      SUBCELLULAR LOCATION: Cytoplasm. Note=Along actomyosin filaments and stress fibers.
      PTM: Phosphorylated by CIT (Rho-associated kinase) (By similarity). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR.
      SIMILARITY: SwissProt: O14974 ## Contains 6 ANK repeats.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated as a substrate for ROKa/ROCK-II, active.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at -20°C
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      12-421 04053252408618

      Documentation

      MYPT1, (714-1004) FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      MYPT1, (714-1004) Certificats d'analyse

      TitreNuméro de lot
      MYPT1 (714-1004) 2477877
      MYPT1 (714-1004) - 2457109 2457109
      MYPT1 (714-1004) (recombinant protein expressed in E. coli) 2947252
      MYPT1 (714-1004) (recombinant protein expressed in E. coli) -2492433 2492433
      MYPT1 (714-1004) - 2533234 2533234
      MYPT1 (714-1004) - 2876675 2876675
      MYPT1 (714-1004) - 2998339 2998339
      MYPT1 (714-1004) - DAM1606765 DAM1606765
      MYPT1 (714-1004) - DAM1651176 DAM1651176
      MYPT1, (714-1004) - 20305 20305
      Show more

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      Catégories

      Life Science Research > Proteins and Enzymes > Substrate & Control Proteins > Kinase & Phosphatase Substrates
      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Kinase & Phosphatase Substrates