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HKI5MAG-99K MILLIPLEX® MAP Human Kidney Injury Magnetic Bead Panel 5 - Toxicity Multiplex Assay

The analytes available for this multiplex kit are: α-1-Microglobulin, Collagen IV, Lipocalin-2/NGAL, Osteoactivin, TIMP-1, Uromodulin.

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HKI5MAG-99K
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Replacement Information
Description
Catalogue NumberHKI5MAG-99K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX® MAP Human Kidney Injury Magnetic Bead Panel 5 - Toxicity Multiplex Assay
OverviewThe MILLIPLEX® MAP Human Kidney Injury Magnetic Bead Panel 5 contains all the components necessary to measure the following six biomarkers in any combination using Luminex® xMAP® technology: α-1-Microglobulin, Collagen IV, Lipocalin-2/NGAL, Osteoactivin, TIMP-1, Uromodulin. The kit uses a 96-well format, contains a lyophilized standard cocktail, two quality controls and can measure up to 38 serum or plasma samples in duplicate.
Background InformationMaintenance of physiological balance in the body is attributed in part to normal kidney function. Altered kidney function due to injury, drug toxicity or kidney failure may be life threatening. Toxicity is the leading cause of drug failure, so research is expanding in search of more sensitive, rapid methods to determine organ-specific damage as quickly as possible. Traditionally, blood urea nitrogen (BUN) and serum creatinine tests have been used to determine drug-induced damage to the kidney. These tests only detect kidney damage after it begins and do not allow for the elucidation of where in the kidney the damage has occurred. Specificity and earlier detection of kidney injury is vital, and this area of research is expanding in search of more sensitive, rapid methods for determining the specific area of damage in kidney.

EMD Millipore provides valuable research assays to investigate multiple biomarkers of kidney injury in human serum and plasma samples using the Luminex xMAP® instrument platform.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Inter-assay: α-1-Microglobulin, Collagen IV, Osteoactivin < 15
  • Inter-assay: Lipocalin-2/NGAL, TIMP-1, Uromodulin < 10
  • Intra-Assay: < 10
Panel TypeMAGNETIC Toxicity
Quality LevelMQ200
Applications
ApplicationThe analytes available for this multiplex kit are: α-1-Microglobulin, Collagen IV, Lipocalin-2/NGAL, Osteoactivin, TIMP-1, Uromodulin.
Key Applications
  • Multiplexing
Application Notes• Customers should determine the optimal dilution factor for their samples. Our recommendations are based on serum and plasma samples from normal subjects.
• This assay requires 25 µL of 1:75 diluted serum or plasma sample per well in kit Assay Buffer (please dilute per protocol instructions).
• An overnight (4°C) incubation is recommended.
Biological Information
SpecificityThere was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.
Species Reactivity
  • Human
Analytes Available
  • α-1-Microglobulin
  • Collagen IV
  • Lipocalin-2/NGAL
  • Osteoactivin
  • TIMP-1
  • Uromodulin
Assay PanelEverything you need in a single kit.
Cross ReactivityThere was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.
Accuracy
  • 62% - 90%
Physicochemical Information
Sensitivity
  • 0.05 - 0.56 ng/mL (MinDC+2SD)
  • Please see kit protocol for individual assay sensitivities.
Linearity of DilutionContact technical support for this information.
Standard Curve Range
  • α-1-Microglobulin: 0.24 – 250 ng/mL
  • Collagen IV: 0.49 – 500 ng/mL
  • Lipocalin-2/NGAL: 0.05 – 50 ng/mL
  • Osteoactivin: 0.20 – 200 ng/mL
  • TIMP-1: 0.10 – 100 ng/mL
  • Uromodulin: 0.05 – 50 ng/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
HKI5MAG-99K 04055977122534

Documentation

Protocols

Title
Protocol- HKI5MAG-99K

MILLIPLEX® MAP Human Kidney Injury Magnetic Bead Panel 5 - Toxicity Multiplex Assay FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

Brochure

Titre
The Power of Biomarker Analysis

Informations techniques

Titre
96 well Template 2 Up
96 well Template Single
HKI5MAG-99K xPonent Protocol tol Template Version Magpix
HKI5MAG-99K xPonent Standard Template Version Magpix
Quality Control Range Sheet- HKI5-106 and HKI5-206

Posters

Titre
Toxicology in drug discovery and development: Biomarkers and model organisms