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CBA013 InnoCyte™ ECM Cell Adhesion Assay, Collagen Type IV

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CBA013
  
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      Aperçu

      Tableau de caractéristiques principal

      Detection Methods
      Fluorometric
      Description
      OverviewA convenient assay for the determination of the relative attachment of adherent cell lines to collagen type IV. Cells are seeded onto the collagen type IV coated plates followed by determination of relative cell attachment using a fluorescent dye.
      Catalogue NumberCBA013
      Brand Family Calbiochem®
      Materials Required but Not Delivered• Pipettors with disposable tips
      • Fluorescence reader capable of measuring fluorescence in 96-well plates with a filter set of excitation wavelength ~485 nm and emission wavelength ~520 nm
      • PBS, preferably containing calcium/magnesium (Dulbecco's PBS) for washing
      References
      ReferencesCaroll, D.K., et al. 2006. Nat. Cell Biol. 8, 551.
      Hynes, R.O., et al. 1999. Proc. Natl. Acad. Sci. USA96, 2588.
      Ohashi, T., et al. 1999. Proc. Natl. Acad. Sci USA 96, 2153.
      Grande, J.P., et al. 1997. J. Lab. Clin. Med. 130, 476.
      Nelson, P.R., et al. 1997. Vasc. Surg. 26, 104.
      Horowitz, A.F., et al. 1996. Trends Cell Biol. 6, 460.
      Hynes, R.O., et al. 1990. Fibronectins, Springer-Verlag, New York.
      Preissner, K.T., et al. 1990. J. Biol. Chem. 265, 18490.
      Product Information
      Detection methodFluorometric
      Form72 Tests
      Format96-well plate
      Kit containsCoated 96-Well Plate, Fluorescent Dye, D-PBS, and a user protocol.
      Applications
      Application ReferencesCaroll, D.K., et al. 2006. Nat. Cell Biol. 8, 551.
      Biological Information
      Assay time2.5 h
      Sample TypeAdherent cultured cells
      Physicochemical Information
      Emission max.
      Excitation max.
      Product Usage Statements
      Intended useThe Innocyte™ Cell Adhesion Microplate Assays are designed for the determination of the relative attachment of adherent cell lines to extracellular matrix proteins such as Human Fibronectin (Cat. No. CBA011), Human Vitronectin (Cat. No. CBA012), and Human Collagen IV (Cat. No. CBA013). Cells are seeded onto a coated substrate, which is followed by the determination of relative cell attachment using a fluorescent dye.
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage Multiple storage requirements
      Storage ConditionsUpon arrival store the Calcein-AM at -20°C and the remaining components at 4°C.
      Protect from Light Protect from light
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Supplemental Information
      Kit containsCoated 96-Well Plate, Fluorescent Dye, D-PBS, and a user protocol.
      Global Trade Item Number
      Référence GTIN
      CBA013 0