Millipore Sigma Vibrant Logo

361523 GSK-3α, GST-Fusion, Human, Recombinant, S. frugiperda

FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.

Synonymes: Glycogen Synthase Kinase 3α

361523
  
Prix en cours de récupération
Le prix n'a pas pu être récupéré
La quantité minimale doit être un multiple de
Maximum Quantity is
À la validation de la commande Plus d'informations
Vous avez sauvegardé ()
 
—
Demander le prix
—
Disponibilité limitée Disponibilité limitée
En stock 
Interrompu(e)
Disponible en quantités limitées
Disponibilité à confirmer
    Pour le restant : Nous vous tiendrons informé
      Pour le restant : Nous vous tiendrons informé
      Nous vous tiendrons informé
      Contacter le Service Clients
      Contact Customer Service

       

      Contacter le Service Clients

      Aperçu

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      Full-length, recombinant, human GSK3α fused at the N-terninus to GST and expressed in S. frugiperda insect cells using a baculovirus expression system. GSK3α is unique in its preference for a priming phosphate at n+4 (where n is the site of phosphorylation by GSK3α) in order to phosphorylate many of its substrates. This motif is found in several well-established substrates of GSK3α, such as glycogen synthase, the epsilon subunit of eIF2B, and ATP citrate-lyase. The active kinase is useful in the study of GSK3α regulation and for inhibitor screening.
      Catalogue Number361523
      Brand Family Calbiochem®
      SynonymsGlycogen Synthase Kinase 3α
      References
      ReferencesMeijer, L., et al. 2004. Trends Pharmacol Sci. 25, 471.
      Patel, S., et al. 2004. Biochem Soc. Trans. 32, 803.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme that will phosphorylate 1 nmol glycogen synthase peptide substrate (YRRAAVPPSPSLSRHSSPHQSEDEEE) per min at 30°C, pH 7.4.
      FormLiquid
      FormulationIn 100 mM NaCl, 25 mM Tris-HCl, 10 mM reduced glutathione, 3 mM DTT, 50% glycerol, 0.05% Tween®-20 detergent, pH 8.0.
      Applications
      Biological Information
      Purity≥80% by SDS-PAGE
      Specific Activity≥700 units/mg protein
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      S PhraseS: 26

      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing intitial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      361523 0

      Documentation

      GSK-3α, GST-Fusion, Human, Recombinant, S. frugiperda Certificats d'analyse

      TitreNuméro de lot
      361523

      Références bibliographiques

      Aperçu de la référence bibliographique
      Meijer, L., et al. 2004. Trends Pharmacol Sci. 25, 471.
      Patel, S., et al. 2004. Biochem Soc. Trans. 32, 803.
      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision27-June-2008 JSW
      SynonymsGlycogen Synthase Kinase 3α
      DescriptionFull-length, recombinant, human GSK3α fused at the N-terninus to GST and expressed in S. frugiperda insect cells using a baculovirus expression system. GSK3α is unique in its preference for a priming phosphate at n+4 (where n is the site of phosphorylation by GSK3α) in order to phosphorylate many of its substrates. This motif is found in several well-established substrates of GSK3α, such as glycogen synthase, the epsilon subunit of eIF2B, and ATP citrate-lyase. The active kinase is useful in the study of GSK3α regulation and for inhibitor screening.
      FormLiquid
      FormulationIn 100 mM NaCl, 25 mM Tris-HCl, 10 mM reduced glutathione, 3 mM DTT, 50% glycerol, 0.05% Tween®-20 detergent, pH 8.0.
      Concentration Label Please refer to vial label for lot-specific concentration
      Recommended reaction conditions
      50 mM HEPES, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 100 µM ATP, 20 µM peptide substrate (YRRAAVPPSPSLSRHSSPHQSEDEEE), 3 µM sodium orthovanadate, and 100 µg enzyme.
      Purity≥80% by SDS-PAGE
      Specific activity≥700 units/mg protein
      Unit definitionOne unit is defined as the amount of enzyme that will phosphorylate 1 nmol glycogen synthase peptide substrate (YRRAAVPPSPSLSRHSSPHQSEDEEE) per min at 30°C, pH 7.4.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing intitial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesMeijer, L., et al. 2004. Trends Pharmacol Sci. 25, 471.
      Patel, S., et al. 2004. Biochem Soc. Trans. 32, 803.