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14-752 GRK7 Protein, active, 10 µg

Active, N-terminal 6His-tagged, recombinant, full length human GRK7 expressed by baculovirus in Sf21 cells. For use in Kinase Assays.

GRK7 Protein, active, 10 µg : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
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14-752
10 µg  
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      Replacement Information
      Description
      Catalogue Number14-752
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionGRK7 Protein, active, 10 µg
      OverviewN-terminal 6His-tagged, recombinant, full length human GRK7 expressed by baculovirus in Sf21 cells.
      References
      Product Information
      Presentation10μg of recombinant protein in 100 μl of 50 mM Tris-HCl pH 8.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
      Quality LevelMQ100
      Applications
      ApplicationActive, N-terminal 6His-tagged, recombinant, full length human GRK7 expressed by baculovirus in Sf21 cells. For use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      SourceRecombinant, full length human GRK7 expressed by baculovirus in Sf21 cells.
      Specific Activity381U/mg, where one unit of GRK7 activity is defined as 1nmol phosphate incorporated into 2mg/ml casein per minute at 30°C with a final ATP concentration of 100μM.
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. It is specifically expressed in the retina and the encoded protein has been shown to phosphorylate cone opsins and initiate their deactivation.
      Gene Symbol
      • GRK7
      • GPRK7
      Protein TargetGRK7
      Purification MethodPurified using Ni2+/NTA agarose. Purity 99% by SDS-PAGE and Coomassie blue staining.
      Target Sub-FamilyAGC
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q8WTQ7 # Phosphorylates cone opsins thereby initiating their deactivation.
      SIZE: 553 amino acids; 62212 Da
      SUBCELLULAR LOCATION: Membrane; Lipid-anchor.
      TISSUE SPECIFICITY: Retina. Cones and rod.
      SIMILARITY: SwissProt: Q8WTQ7 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 protein kinase domain. & Contains 1 RGS domain.
      Molecular Weight66 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by phosphorylation of 2 mg/mL casein.
      Purity was assessed by SDS-PAGE and Coomassie blue staining using 3μg of GRK7.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
      Packaging Information
      Material Size10 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      14-752 04053252516214

      Produits & Applications associés

      Alternative Packsize

      Référence Description  
      14-752M GRK7 Protein, active, 250 µg Prix & Disponibilité

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      Catégories

      Life Science Research > Proteins and Enzymes > Purified Kinases
      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases