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SCC611 GRINCH Glucose-Responsive Insulin-secreting C-peptide hProinsulin Rat Insulinoma Cell Line

SCC611, the GRINCH rat insulinoma cell line, is a pancreatic beta cell line transfected with the hPro-CpepSfGFP construct containing the human insulin sequence.

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≥1 X10⁶ cells/vial   
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      Replacement Information
      Description
      Catalogue NumberSCC611
      DescriptionGRINCH Glucose-Responsive Insulin-secreting C-peptide hProinsulin Rat Insulinoma Cell Line
      OverviewGRINCH (Glucose-Responsive Insulin-secreting C-peptide modified Human proinsulin) is a rat insulinoma cell line derived from the INS-1 cell line. INS-1 cells were transfected with the hPro-CpepSfGFP construct containing the human insulin sequence with a C-peptide modified with a C-terminal superfolder GFP insert. The GRINCH cell line exhibits efficient glucose-stimulated insulin secretion, making it a good model to study insulin secretion regulation and pancreatic β-cell function. The fluorescent C-peptide is co-stored and co-secreted with human insulin, allowing the tracking and quantification of insulin biosynthesis in real time.

      GRINCH cells express human insulin with a C-peptide GFP tag​.
      Alternate Names
      • Glucose-Responsive INsulin-secreting C-peptide-modified cell line
      • Rat insulinoma cell line
      • Insulinoma cell line
      Background InformationApproximately 1 in 11 adults globally has diabetes, leading to 12% of health expenditure being diabetes- related. Diabetes mellitus refers to a group of metabolic diseases involving chronic hyperglycemia due to reduced insulin secretion, insulin synthesis, or increased insulin resistance.

      Recent advancements in technology have enabled the molecular understanding of diabetes in childhood and in non-obese patients, unveiling the role of single gene defects. These defects, found in genes encoding beta-cell components like potassium channels, glucokinase, transcription factors, and insulin, contribute to 1-5% of all diabetes cases and are characteristic of monogenic diabetes. Insulin gene mutations can lead to various diabetes subtypes by affecting insulin folding, causing retention in the endoplasmic reticulum (ER), triggering ER stress, beta-cell death, and impairing insulin-insulin receptor (IR) interaction.

      Source

      The GRINCH cell line was derived from INS-1 cells. The INS-1 cell line was derived from x-ray induced insulinoma in rats.

      References

      1. Trikkalinou A, Papazafiropoulou AK, Melidonis A. 2017. Type 2 diabetes and quality of life. World Journal of Diabetes. 8(4):120.
      2. Yang Y, Chan L. 2016. Monogenic Diabetes: What It Teaches Us on the Common Forms of Type 1 and Type 2 Diabetes. Endocrine Reviews. 37(3):190–222.
      3. Haataja L, Snapp EL, Wright JJ, Li M, Hardy A, Wheeler MB, Markwardt ML, Rizzo MA, Arvan P. 2013. Proinsulin Intermolecular Interactions during Secretory Trafficking in Pancreatic β Cells. 288(3):1896–1906.
      References
      Product Information
      Components
      • ≥1x106 viable GRINCH Glucose-Responsive Insulin-secreting C-peptide hProinsulin Rat Insulinoma Cells: (Catalog No. SCC611). Store in liquid nitrogen.
      Quality LevelMQ100
      Applications
      ApplicationSCC611, the GRINCH rat insulinoma cell line, is a pancreatic beta cell line transfected with the hPro-CpepSfGFP construct containing the human insulin sequence.
      Key Applications
      • Cell Culture
      • Cell Function Assay
      Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@milliporesigma.com.
      Biological Information
      HostRat
      Cell Line Type
      • Insulinoma
      • Pancreas
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance• Each vial contains ≥ 1 X106 viable cells.
      • Cells are tested negative for infectious diseases by Essential CLEAR Panel by Charles River Animal Diagnostic Services.
      • Cells are verified to be of rat origin and negative for interspecies contamination from human, mouse, Chinese hamster, Golden Syrian hamster, and nonhuman primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services
      • Cells are negative for mycoplasma contamination.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Storage and Shipping Information
      Storage ConditionsGRINCH Glucose-Responsive Insulin-secreting C-peptide hProinsulin Rat Insulinoma cells should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages without significantly affecting cell marker expression and function.
      Packaging Information
      Material Size≥1 X10⁶ cells/vial
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      SCC611 04065271047716