362331 Sigma-AldrichGAG Agonist, Adhesamine - Calbiochem

This product has been discontinued.

A symmetrical nonpeptidyl cationic diaryldispirotripiperazine compound that binds GAGs (glycosaminoglycans) in a reversible manner (K_D = 0.39, 4.67, and 5.85 µM for heparin, heparan sulfate, and keratin sulfate, respectively) and effectively promotes adhesion (by ~100 and 600% at 60 µM, respectively) and growth (by ~80 and 50% in 72 h at 1 µM, respectively) of HepG2 and Jurkat cells in a dose-dependent manner. Binding studies using heparin-derived oligosaccharides indicate that the major mode of interaction involves the binding of a hexasaccharide unit by two adhesamine molecules (K_D = 0.12 µM). Primary murine hippocampal neurons cultured in the presence of adhesamine are reported to exhibit earlier differentiation as well as improved survival rate when compared with neurons cultured on poly-L-lysine-coated surface. A GAGs binding antagonist Surfen is also available under Cat. No. 362330.