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324708 Endoproteinase Asp-N, Excision Grade, Pseudomonas fragi

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324708
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324708-2UG
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      Description
      OverviewNative endoproteinase Asp-N from Pseudomonas fragi. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Metalloprotease that specifically hydrolyzes proteins at the N-terminal side of aspartic acid and cysteic acid residues. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.
      Catalogue Number324708
      Brand Family Calbiochem®
      References
      ReferencesDrapeau, G.R. 1980. J. Biol. Chem. 255, 839.
      Product Information
      CAS number9001-92-7
      Unit of DefinitionOne unit is defined as the amount of enzyme that will release a sufficient amount of azo dye from AZOCOLL™ Substrate (Cat. No. 194932) to produce an increase in absorbance at 520 nm of 0.001 per min at 37°C, pH 7.5.
      EC number3.4.24.33
      FormWhite lyophilized solid
      FormulationLyophilized from 10 mM Tris-HCl, pH 7.5.
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥90% by SDS-PAGE
      Specific Activity≥20,000 units/mg protein
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      R PhraseR: 20-36/37/38-42/43

      Harmful by inhalation.
      Irritating to eyes, respiratory system and skin.
      May cause sensitization by inhalation and skin contact.
      S PhraseS: 22-26-36

      Do not breathe dust.
      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Harmful
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      324708-2UG 04055977215939

      Documentation

      Endoproteinase Asp-N, Excision Grade, Pseudomonas fragi FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Endoproteinase Asp-N, Excision Grade, Pseudomonas fragi Certificats d'analyse

      TitreNuméro de lot
      324708

      Références bibliographiques

      Aperçu de la référence bibliographique
      Drapeau, G.R. 1980. J. Biol. Chem. 255, 839.
      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision19-June-2008 RFH
      DescriptionNative endoproteinase Asp-N from Pseudomonas fragi. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Metalloprotease that specifically hydrolyzes proteins and the N-terminal side of aspartic acid and cysteic acid residues. Inhibited by aprotinin, DFP, leupeptin, and TLCK.
      FormWhite lyophilized solid
      FormulationLyophilized from 10 mM Tris-HCl, pH 7.5.
      Recommended reaction conditions
      Protocol 1. Reconstitute in 50 µl of distilled H2O. 2. Dissolve the proteins to be sequenced in phosphate, acetate, or Tris buffer, pH 6.0-8.5. For proteins that are difficult to solubilize, add urea (up to 1 M), SDS (0.01%), or guanidine-HCl (up to 1 M) to the digestion buffer. 3. Use a 1:20-1:100 ratio of proteinase to protein by weight for sequence analysis. 4. Incubate between 2-18 h at 37°C. Incubation time is dependent on the amount of enzyme used.
      CAS number9001-92-7
      EC number3.4.24.33
      Purity≥90% by SDS-PAGE
      Specific activity≥20,000 units/mg protein
      Unit definitionOne unit is defined as the amount of enzyme that will release a sufficient amount of azo dye from AZOCOLL™ Substrate (Cat. No. 194932) to produce an increase in absorbance at 520 nm of 0.001 per min at 37°C, pH 7.5.
      SolubilityH₂O
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.
      Toxicity Harmful
      ReferencesDrapeau, G.R. 1980. J. Biol. Chem. 255, 839.

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      Catégories

      Life Science Research > Proteins and Enzymes > Other Enzymes