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14-476 Cdk7/Cyclin H/MAT1 (CAK complex) Protein, active, 10 µg

Active complex of recombinant full length human Cdk7, containing a C-terminal His6-tag, recombinant full-length human Cyclin H untagged & recombinant full-length human MAT1 containing an N-terminal GST-tag, for use in Kinase Assays.

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14-476
10 µg  Also available in 250μg size, please inquire for pricing and availability.
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      Replacement Information
      Description
      Catalogue Number14-476
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionCdk7/Cyclin H/MAT1 (CAK complex) Protein, active, 10 µg
      OverviewComplex of recombinant full length human cdk7, containing a C-terminal His6-tag, recombinant full-length human cyclin H untagged and recombinant full-length human MAT1 containing an N-terminal GST-tag
      References
      Product Information
      Quality LevelMQ100
      Applications
      ApplicationActive complex of recombinant full length human Cdk7, containing a C-terminal His6-tag, recombinant full-length human Cyclin H untagged & recombinant full-length human MAT1 containing an N-terminal GST-tag, for use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      SourceAll are expressed by baculovirus in Sf21 insect cells
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This protein forms a trimeric complex with cyclin H and MAT1, which functions as a Cdk-activating kinase (CAK). It is an essential component of the transcription factor TFIIH, that is involved in transcription initiation and DNA repair. This protein is thought to serve as a direct link between the regulation of transcription and the cell cycle.
      Gene Symbol
      • CDK7
      • p39MO15
      • CAK
      • CAK1
      • MO15
      • CDKN7
      • STK1
      Protein TargetCDK7/cyclinH/MAT1
      Purification MethodNi2+/NTA-agarose
      Target Sub-FamilyCMGC
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P50613 # Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between two subsequent phases in the cell cycle. CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex, a serine-threonine kinase. CAK activates the cyclin-associated kinases CDC2/CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.
      SIZE: 346 amino acids; 39038 Da
      SUBUNIT: Associates primarily with cyclin H and MAT1 to form the CAK complex. CAK can further associate with the core-TFIIH to form the TFIIH basal transcription factor. Interacts with PUF60.
      SUBCELLULAR LOCATION: Nucleus.
      TISSUE SPECIFICITY: Ubiquitous.
      PTM: Phosphorylation of Ser-164 during mitosis inactivates the enzyme. & Phosphorylation of Thr-170 is required for activity.
      SIMILARITY: SwissProt: P50613 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. & Contains 1 protein kinase domain.
      Molecular Weight39.9kDa (cdk7), 37.8kDa (cyclin H), 62.8kDa (MAT1).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by phosphorylation of cdk7 substrate peptide
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at -70°C
      Packaging Information
      Material Size10 µg
      Material PackageAlso available in 250μg size, please inquire for pricing and availability.
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      14-476 04053252373541

      Documentation

      Cdk7/Cyclin H/MAT1 (CAK complex) Protein, active, 10 µg FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Cdk7/Cyclin H/MAT1 (CAK complex) Protein, active, 10 µg Certificats d'analyse

      TitreNuméro de lot
      Cdk7/cyclinH/MAT1, active - 1906800-B 1906800-B
      Cdk7/cyclinH/MAT1, active - D8HN020U-F D8HN020U-F
      Cdk7/cyclinH/MAT1, active - D8HN020U-G D8HN020U-G
      Cdk7/cyclinH/MAT1, active -WAB0365-B WAB0365-B

      FAQ

      QuestionRéponse
      How is this complex produced?It is a coexpressed protein - the cells are hit with all three viruses and the complex is purified via the his-tagged CDK - there is no invitro reconstitution. We are not able to comment if there are higher-order dimers (one complex dimerising with another).
      What type of buffer is best for lysis when attempting to preserve a protein?The best buffer is Buffer A. It is composed of 50mM Tris/HCl, pH 7.5, 1mM EGTA, 1mM EDTA, 1mM Sodium Orthovanadate (activated), 10mM Na B-glycerolphosphate, 50mM NaF, 5mM Na pyrophosphate, 1% w/v Triton X-100, 0.1% w/v 2-mercaptoethanol, 1ug/ml each of aprotinin, leupeptin and pepstatin, 1mM PMSF and 1mM microcystin (this is optional). QS with water. You may store Buffer A in aliquots at -20°C for 6 months.

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      Alternative Packsize

      Référence Description  
      14-476M Cdk7/Cyclin H/MAT1 (CAK complex) Protein, active, 250 µg Prix & Disponibilité

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      Catégories

      Life Science Research > Proteins and Enzymes > Purified Kinases
      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases