Millipore Sigma Vibrant Logo

SCT203 BioTracker™ Orange Zymosan Phagocytosis Live Cell Probe

This live cell imaging probe is zymosan conjugated to fluorescent orange dye & can be used to detect phagocytosis using fluorescence microscopy or flow cytometry.

SCT203
1 mg  
Prix en cours de récupération
Le prix n'a pas pu être récupéré
La quantité minimale doit être un multiple de
Maximum Quantity is
À la validation de la commande Plus d'informations
Vous avez sauvegardé ()
 
—
Demander le prix
—
Disponibilité limitée Disponibilité limitée
En stock 
Interrompu(e)
Disponible en quantités limitées
Disponibilité à confirmer
    Pour le restant : Nous vous tiendrons informé
      Pour le restant : Nous vous tiendrons informé
      Nous vous tiendrons informé
      Contacter le Service Clients
      Contact Customer Service

       

      Contacter le Service Clients

      Aperçu

      Replacement Information
      Description
      Catalogue NumberSCT203
      Erreur de configuration des données. Pagelet <feature_brand-BioTracker> non trouvé.
      DescriptionBioTracker™ Orange Zymosan Phagocytosis Live Cell Probe
      OverviewZymosan A is a compound prepared from yeast cell walls and commonly used to induce inflammatory responses, especially phagocytosis. BioTracker™ Orange Zymosan Phagocytosis Live Cell Probe is Zymosan A labeled with an orange acidic pH indicator.

      The fluorescence of this indicator is especially enhanced in an acidic environment such as that found in phagosomes or other acidic organelles. SCT203 is therefore a useful probe for the observaton of phagocytosis via its fluorescence increase, which can be only observed after phagocytosis. This probe emits fluorescence when excited by 532 or 514 nm sources, in addition to others such as the 488 laser that are compatible with the excitation range.

      Spectral Properties
      Absorbance (max): 535 nm
      Emission (max): 568 nm (Orange)
      Alternate Names
      • Live Cell Imaging Probe
      • Fluorescent Zymosan Probe
      • orange zymosan
      • orange zymosan live cell probe
      Background InformationZymosan A is a compound prepared from yeast cell walls that is frequently used to induce inflammatory responses, including phagocytosis. SCT203 is Zymosan A labeled with AcidiFluor ORANGE, an acid pH indicator. Its fluorescence is enhanced in the acidic environment of the phagosome. BioTracker™ Orange Zymosan Phagocytosis Live Cell Probe is therefore a useful tool for the detection of the acidified conditions that follow phagocytosis.
      References
      Product Information
      Detection methodFluorescence Flow cytometry
      FormatLyophilized solid
      PresentationReddish-purple solid
      Quality LevelMQ100
      Applications
      ApplicationThis live cell imaging probe is zymosan conjugated to fluorescent orange dye & can be used to detect phagocytosis using fluorescence microscopy or flow cytometry.
      Key Applications
      • Cell Based Assays
      • Flow Cytometry
      • Fluorescence
      Application NotesThis fluorescently-labeled zymosan-A enables fluorescent imaging of phagocytic cells performing phagocytosis.

      The lyophilized probe may be reconstituted in culture medium to 10 to 50 ug/mL, but concentration should be optimized to the user's application.

      SCT203 has been used to analyze phagocytosis by fluorescence microscopy or flow cytometry in overnight-cultured RAW264.7 cells after incubation for 1-4h.
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore BioTracker™ Orange Zymosan Phagocytosis Live Cell Probe at -20°C, desiccated and protected from light. Centrifuge vial briefly to collect contents at bottom of vial before opening. Storing as a solution is not recommended.
      Packaging Information
      Material Size1 mg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      SCT203 04065269254249

      Documentation