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AB9986 Anti-phospho-BRCA2 (Ser3291) Antibody

Anti-phospho-BRCA2 (Ser3291) Antibody is a rabbit polyclonal antibody for detection of phospho-BRCA2 (Ser3291), also known as BRCA1/BRCA2-containing complex subunit 2 & Fanconi anemia group D1 pro

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Anti-phospho-BRCA2 (Ser3291) Antibody : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Research Category: Epigenetics & Nuclear Function, Epigenetics & Nuclear Function Research Sub-Category: Cell Cycle, DNA Replication & Repair, Oncoproteins Gene Symbol: BRCA2, BRCC2, BROVCA2, FACD, FAD, FAD1, FANCB, FANCD, FANCD1 UniProt Number: P51587
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AB9986
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      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Eq, M, R, Chp, Ca, Ht, Rhesus MacaqueICC, IHC, DBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB9986
      DescriptionAnti-phospho-BRCA2 (Ser3291) Antibody
      Alternate Names
      • BRCA1/BRCA2-containing complex, subunit 2
      • Fanconi anemia group D1 protein 3
      • Fanconi anemia, complementation group D1
      • breast and ovarian cancer susceptibility gene, early onset
      • breast cancer 2 tumor suppressor
      • breast cancer 2, early onset
      • breast cancer susceptibility protein BRCA2
      Background InformationBRCA2 (Breast Cancer Type 2 susceptibility protein) is a tumor suppressor protein encoded by the BRCA2 gene.
      The protein encoded by this gene plays a direct role in error-free double-strand break repair and homologous recombination. BRCA2 shares similarities with the tumor suppressor gene BRCA1. Inherited mutations in BRCA2 as well as BRCA1 are associated with an increased risk of developing breast or ovarian cancer. Although the structures of the BRCA1 and BRCA2 genes are very different, some functions are similar. The proteins made by both genes are essential for repairing damaged DNA and interact with the protein produced by the RAD51 gene. Together these three proteins play a role in maintaining the stability of the human genome and prevent dangerous gene rearrangements that can lead to the development of cancer.
      Product Information
      FormatAffinity Purified
      Control
      • Modified and non modified peptides of phosphorylated and non-phosphorlyated BRCA2
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-phospho-BRCA2 (Ser3291) Antibody is a rabbit polyclonal antibody for detection of phospho-BRCA2 (Ser3291), also known as BRCA1/BRCA2-containing complex subunit 2 & Fanconi anemia group D1 protein 3, has been validated in ICC, IHC, DB.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Dot Blot
      Application NotesImmunocytochemistry Analysis: 1:500 dilution from a previous lot detected BRCA2 in MCF-7 cells.

      Immunohistochemistry Analysis: 1:100 dilution from a previous lot detected BRCA2 in metastatic ductal carcinoma tissue.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human BRCA2 phosphorylated at and around the internal domain surrounding Ser3291.
      EpitopeInternal Domain surrounding Ser3291
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes human BRCA2 phosphorylated at and around the internal domain surrounding Ser3291.
      Species Reactivity
      • Human
      • Horse
      • Mouse
      • Rat
      • Chimpanzee
      • Canine
      • Hamster
      • Rhesus Macaque
      Species Reactivity NoteDemonstrated to react with human. Predicted to react with equine, mouse, rat, chimpanzee, canine, hamster, rhesus monkey, and cat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryInherited mutations in BRCA1 and this gene, BRCA2, confer increased lifetime risk of developing breast or ovarian cancer. Both BRCA1 and BRCA2 are involved in maintenance of genome stability, specifically the homologous recombination pathway for double-strand DNA repair. The BRCA2 protein contains several copies of a 70 aa motif called the BRC motif, and these motifs mediate binding to the RAD51 recombinase which functions in DNA repair. BRCA2 is considered a tumor suppressor gene, as tumors with BRCA2 mutations generally exhibit loss of heterozygosity (LOH) of the wild-type allele. [provided by RefSeq].
      Gene Symbol
      • BRCA2
      • BRCC2
      • BROVCA2
      • FACD
      • FAD
      • FAD1
      • FANCB
      • FANCD
      • FANCD1
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Involved in double-strand break repair and/or homologous recombination. May participate in S phase checkpoint activation.

      SIZE: 3418 amino acids; 384225 Da

      SUBUNIT: Interacts with RAD51 and DSS1. Interacts with ubiquitinated FANCD2. Interacts with PALB2, enables the recombinational repair and checkpoints functions. Interacts with WDR16.

      TISSUE SPECIFICITY: Highest levels of expression in breast and thymus, with slightly lower levels in lung, ovary and spleen.

      PTM: Phosphorylated by ATM upon irradiation-induced DNA damage.

      DISEASE: Defects in BRCA2 are a cause of genetic susceptibility to breast cancer (BC) [MIM:600185, 114480]. BC is an extremely common malignancy, affecting one in eight women during their lifetime. A positive family history has been identified as major contributor to risk of development of the disease, and this link is striking for early-onset breast cancer. Mutations in BRCA2 are thought to be responsible for some inherited breast cancer. It is linked with male breast cancer. & Defects in BRCA2 are the cause of Fanconi anemia complementation group D type 1 (FANCD1) [MIM:605724]. Fanconi anemia [MIM:227650] is an autosomal recessive disorder affecting all bone marrow elements and associated with cardiac, renal, and limb malformations as well as dermal pigmentary changes.

      SIMILARITY: Contains 8 BRCA2 repeats.
      Molecular Weight~ 390 kDa
      Product Usage Statements
      Quality AssuranceEvaluated by Dot Blot in modified and non modified peptides of phosphorylated and non-phosphorlyated BRCA2.

      Dot Blot Analysis: 2 µg/mL of this antibody detected BRCA2 in modified and non modified peptides of phosphorylated and non-phosphorlyated BRCA2.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Référence GTIN
      AB9986 04053252672262