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MABE1073 Anti-acetyl SMC3 Antibody (Lys105/106), clone 21A7

Anti-acetyl SMC3 (Lys105/106), clone 21A7, Cat. No. MABE1073, is a highly specific mouse monoclonal antibody that targets SMC3 Lys105/106 acetylation and has been tested in Chromatin Immunoprecipitation (ChIP), ChIP-seq, ELISA, and Western Blotting.

FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Research Category: Epigenetics & Nuclear Function Gene Symbol: SMC3, BAM, BMH, CDLS3, CSPG6, SMC3L1 UniProt Number: Q9UQE7
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MABE1073
100 µL  
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      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HChIP, ChIP-seq, ELISA, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE1073
      DescriptionAnti-acetyl SMC3 Antibody (Lys105/106), clone 21A7
      Alternate Names
      • Structural maintenance of chromosomes protein 3, Lys105/106 acetylated
      • Bamacan, Lys105/106 acetylated
      • Basement membrane-associated chondroitin proteoglycan, Lys105/106 acetylated
      • Chondroitin sulfate proteoglycan 6, Lys105/106 acetylated
      • Chromosome-associated polypeptide, Lys105/106 acetylated
      • hCAP, Lys105/106 acetylated
      • SMC protein 3, Lys105/106 acetylated
      • SMC-3, Lys105/106 acetylated
      Background InformationStructural maintenance of chromosomes protein 3 (UniProt Q9UQE7; also known as Bamacan, Basement membrane-associated chondroitin proteoglycan, Chondroitin sulfate proteoglycan 6, Chromosome-associated polypeptide, hCAP, SMC protein 3, SMC-3) is encoded by the SMC3 (also known as BAM, BMH, CDLS3, CSPG6, SMC3L1) gene (Gene ID 9126) in human. Cohesion is a nuclear tripartite complex composed of Rad21 and two structural maintenance of chromosomes proteins, SMC1 and SMC3. Cohesin plays an important role in sister chromatid cohesion, as well as in DNA repair and gene expression regulation. To establish sister chromatid cohesion during S phase, SMC3 is acetylated (on K105 and K106 in human) by two cohesin acetyltransferases (CoATs), ESCO1 and ESCO2, that differ in their N-terminal domains and expression during development and across the cell cycle. ChIP-seq analysis reveals that ESCO1 is recruited by cohesin to over 11,000 chromatin sites occupied by cohesin and CTCF that mediate long-range chromatin interactions and regulate transcription globally. On ther other hand, ESCO2 is infrequently enriched at sites targeted by REST/NRSF (RE1-silencing transcription factor/neuron-restrictive silencer factor) that represses transcription of neuron-specific genes. SMC3 acetylation is thought to induce conformational changes of the dimeric hinge structure during S phase and to allow dimer opening and loading of DNA.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-acetyl SMC3 (Lys105/106), clone 21A7, Cat. No. MABE1073, is a highly specific mouse monoclonal antibody that targets SMC3 Lys105/106 acetylation and has been tested in Chromatin Immunoprecipitation (ChIP), ChIP-seq, ELISA, and Western Blotting.
      Key Applications
      • Chromatin Immunoprecipitation (ChIP)
      • ChIP-seq
      • ELISA
      • Western Blotting
      Application NotesELISA Analysis: A representative lot bound Lys105/106-diacetylated SMC3 peptide with 2-fold higher affinity than Lys105-monoacetylated SMC3 peptide (Kd = 0.04 and 0.09 µg/mL, respectively), while displaying much reduced affinity toward Lys105-monoacetylated (Kd = 0.73 µg/mL) and little affinity toward nonacetylated (Kd = 2.99 µg/mL) peptide (Rahman, S., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(36):11270-11275).

      Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected enhanced SMC3 occupancy at target chromatin sites in HDAC8-null HCT116 cells than wild-type HCT-116 cells (Rahman, S., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(36):11270-11275).

      ChIP-seq Analysis: A representative lot detected SMC3 chromosome 11 enrichment sites, including 81% of Rad21 target sites and 70% of Esco1 target sites, by ChIP-seq (Rahman, S., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(36):11270-11275).

      Western Blotting Analysis: A representative lot detected SMC3 Lys105/106 acetylation in HeLa cells. ESCO1 or ESCO2 depletion by shRNA treatment reduced SMC3 Lys105/106 acetylation level, while dual ESCO1/2 depletion resulted in most profound SMC3 Lys105/106 acetylation reduction (Rahman, S., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(36):11270-11275).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the target region sequence of human/mouse/rat SMC3 containing acetylated Lys105 and Lys106.
      Clone21A7
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 21A7 bound Lys105/106-diacetylated SMC3 peptide with 2-fold higher affinity than Lys105-monoacetylated SMC3 peptide (Kd = 0.04 and 0.09 µg/mL, respectively), while displaying much reduced affinity toward Lys105-monoacetylated (Kd = 0.73 µg/mL) and little affinity toward nonacetylated (Kd = 2.99 µg/mL) peptide (Rahman, S., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(36):11270-11275).
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Bovine, Mouse, Non-Human Primate, Rat, Xenopus based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SMC3
      • BAM
      • BMH
      • CDLS3
      • CSPG6
      • SMC3L1
      Modifications
      • Acetylation
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~160 kDa observed. 142.0/141.5/141.6/138.4/140.7 kDa (bovine/human/mouse/rat/xenopus) calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human retinal epithelial pigment (REP) cells.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected a reduced SMC3 Lys105/106 acetylation in 10 µg of lysate from N-acetyltransferase ESCO1-knockout human retinal epithelial pigment (REP) cells than in lysate from wild-type REP cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      MABE1073 04054839083686

      Documentation

      Anti-acetyl SMC3 Antibody (Lys105/106), clone 21A7 FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Anti-acetyl SMC3 Antibody (Lys105/106), clone 21A7 Certificats d'analyse

      TitreNuméro de lot
      Anti-acetyl SMC3 (Lys105/106), -Q2767827 Q2767827
      Anti-acetyl SMC3 (Lys105/106), clone 21A7 - 2895552 2895552
      Anti-acetyl SMC3 (Lys105/106), clone 21A7 - 3068067 3068067
      Anti-acetyl SMC3 (Lys105/106), clone 21A7 - 3244387 3244387
      Anti-acetyl SMC3 (Lys105/106), clone 21A7 - 3540378 3540378
      Anti-acetyl SMC3 (Lys105/106), clone 21A7 - 3703565 3703565
      Anti-acetyl SMC3 (Lys105/106), clone 21A7 - 3794315 3794315
      Anti-acetyl SMC3 (Lys105/106), clone 21A7 - 3850516 3850516
      Anti-acetyl SMC3 (Lys105/106), clone 21A7 - 3990924 3990924
      Anti-acetyl SMC3 (Lys105/106), clone 21A7 - 4123895 4123895
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