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662106 Anti-Ubiquitin-Activating Enzyme E1A/E1B Rabbit pAb

FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.

Synonymes: Anti-E1A/E1B, C-terminal

662106
  
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      Aperçu

      Replacement Information

      Tableau de caractéristiques principal

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~117 kDa E1A and the ~110 kDa E1B ubiquitin activating enzymes.
      Catalogue Number662106
      Brand Family Calbiochem®
      SynonymsAnti-E1A/E1B, C-terminal
      References
      ReferencesShang, F., et al. 1997. J. Biol. Chem. 272, 23086.
      Stephen, A.G., et al. 1997. J. Biol. Chem. 272, 10895.
      Stephen, A.G., et al. 1996. J. Biol. Chem. 271, 15608.
      Product Information
      FormLiquid
      FormulationUndiluted serum.
      PreservativeNone
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1:500)
      Application CommentsThe antibody specifically reacts with human E1A and E1B. Does not cross-react with other proteins by immunoblotting. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogena synthetic peptide (KHVRALVLELCCNDESGEDVEVPYVRYTIR) corresponding to amino acids from a common C-terminal sequence of human E1A and E1B
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      662106 0

      Documentation

      Anti-Ubiquitin-Activating Enzyme E1A/E1B Rabbit pAb FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Anti-Ubiquitin-Activating Enzyme E1A/E1B Rabbit pAb Certificats d'analyse

      TitreNuméro de lot
      662106

      Références bibliographiques

      Aperçu de la référence bibliographique
      Shang, F., et al. 1997. J. Biol. Chem. 272, 23086.
      Stephen, A.G., et al. 1997. J. Biol. Chem. 272, 10895.
      Stephen, A.G., et al. 1996. J. Biol. Chem. 271, 15608.

      Brochure

      Titre
      Caspases and other Apoptosis Related Tools Brochure
      Proteasomes Technical Bulletin
      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision16-August-2007 RFH
      SynonymsAnti-E1A/E1B, C-terminal
      ApplicationImmunoblotting (1:500)
      DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~117 kDa E1A and the ~110 kDa E1B ubiquitin activating enzymes.
      BackgroundUbiquitinylation, the covalent attachment of ubiquitin to protein, regulates a number of cellular processes including responses to stress. This process is responsible for selective targeting of proteins for degradation. The first step in ubiquitinylation is the activation of ubiquitin by ubiquitin activating enzyme E1 which is a rate-limiting enzyme in the conjugation process. The ubiquitin-activating enzyme exists as two isoforms: E1A (~117 kDa), localized predominantly in the nucleus, and E1B (~110 kDa), localized in the cytoplasm. The first 11 amino acids are considered to be essential for phosphorylation and exclusive nuclear localization of the enzyme.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide (KHVRALVLELCCNDESGEDVEVPYVRYTIR) corresponding to amino acids from a common C-terminal sequence of human E1A and E1B
      IsotypeIgG
      Specieshuman
      FormLiquid
      FormulationUndiluted serum.
      PreservativeNone
      CommentsThe antibody specifically reacts with human E1A and E1B. Does not cross-react with other proteins by immunoblotting. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesShang, F., et al. 1997. J. Biol. Chem. 272, 23086.
      Stephen, A.G., et al. 1997. J. Biol. Chem. 272, 10895.
      Stephen, A.G., et al. 1996. J. Biol. Chem. 271, 15608.