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MABT1336-25UL Anti-Tropomyosin 3 alpha chain Antibody, clone 4D10A5

Anti-Tropomyosin 3 alpha chain, clone 4D10A5, Cat. No. MABT1336, is a mouse monoclonal antibody that detects Tropomyosin alpha-3 chain and is tested for use in Western Blotting.

MABT1336-25UL
25 µL  
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      Aperçu

      Replacement Information
      Description
      Catalogue NumberMABT1336-25UL
      DescriptionAnti-Tropomyosin 3 alpha chain Antibody, clone 4D10A5
      Alternate Names
      • Tropomyosin alpha-3 chain
      • Gamma-tropomyosin
      • Tropomyosin-3
      • Tropomyosin-5
      • Tpm3
      Background InformationTropomyosin alpha-3 chain (UniProt: Q63610; also known as Gamma-tropomyosin, Tropomyosin-3, Tropomyosin-5, Tpm3) is encoded by the Tpm3 (also known as Tpm-5, Tpm5) gene (Gene ID: 117557) in rat. Tropomyosins are rod-like dimers of coiled-coil proteins that provide stability to actin filaments and regulate access of other actin-binding proteins. In muscle cells, they regulate muscle contraction by controlling the binding of myosin heads to the actin filament. Tropomyosin-3 is a homodimeric protein in slow-twitch skeletal muscle that can also form heterodimers of an alpha (TPM1, TPM3 or TPM4) and a beta (TPM2) chain. It binds to actin filaments in muscle and non-muscle cells and plays crucial role in the calcium dependent regulation of vertebrate striated muscle contraction. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. Mutations in Tpm3 gene are associated with diseases of skeletal muscle such as nemaline myopathy, distal arthrogryposis, and the cap disease that is characterized by myopathy and hypotonia that progresses over time. Clone 4D10A5 recognizes the mammalian tropomyosin exon 9a present in muscle and cytoskeletal isoforms produced by the TPM1, TPM2 and TPM3 genes. (Ref.: Schevzov, G., et al. (2011). BioArchitecture, 1 (4); 135-164).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Tropomyosin 3 alpha chain, clone 4D10A5, Cat. No. MABT1336, is a mouse monoclonal antibody that detects Tropomyosin alpha-3 chain and is tested for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected Tropomyosin 3 alpha chain in Western Blotting applications (Schevzov, G., et. al. (2011). Bioarchitecture. 1(4):135-164).

      Western Blotting Analysis: A 1:125-500 dilution from a representative lot detected Tropomyosin 3 alpha chain in Mouse kidney, skeletal muscle, and recombinant 9a samples (Courtesy of Dr. Jeff Hook, Ph.D., School of Medical Sciences, UNSW Sydney Australia).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenDiphtheria Toxoid Carrier Protein conjugated linear peptide corresponding to 27 amino acids from the C-terminal region of isoform 2 of rat Tropomyosin alpha-3 chain (Tpm 3).
      EpitopeC-terminal
      Clone4D10A5
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 4D10A5 is a mouse monoclonal antibody that detects Tropomyosin alpha-3 (Tpm3) chain of isoform 2. It targets and epitope within 27 amino acids from the C-terminal region.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteHuman, Mouse, Rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Tpm3
      • Tpm-5
      • Tpm5
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight28.72 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceIsotype testing: Identity Confirmation by Isotyping Test.



      Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG2b.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      MABT1336-25UL 04061842378036

      Documentation

      Anti-Tropomyosin 3 alpha chain Antibody, clone 4D10A5 FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Anti-Tropomyosin 3 alpha chain Antibody, clone 4D10A5 Certificats d'analyse

      TitreNuméro de lot
      Anti-Tropomyosin 3 alpha chain, clone 4D10A5 - Q3484346 Q3484346

      Références bibliographiques

      Aperçu de la référence bibliographiqueNº PubMed
      Tropomyosin isoforms and reagents
      Galina Schevzov 1 , Shane P Whittaker, Thomas Fath, Jim Jc Lin, Peter W Gunning
      Bioarchitecture  1(4)  135-164  2010

      Afficher le résumé       		22069507 22069507