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MABF2156-100UL Anti-TLR9 Antibody, clone NaR9

Anti-TLR9, clone NaR9, Cat. No. MABF2156, is a mouse monoclonal antibody that detects Toll-like receptor 9 and has been tested for use in Flow Cytometry, Immunocytochemistry, and Inhibition/ Function Analysis.

MABF2156-100UL
100 µL  
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      Aperçu

      Replacement Information
      Description
      Catalogue NumberMABF2156-100UL
      DescriptionAnti-TLR9 Antibody, clone NaR9
      Alternate Names
      • Toll-like receptor 9
      • CD289
      Background InformationToll-like receptor 9 (UniProt: Q9EQU3; also known as CD289) is encoded by the Tlr9 gene (Gene ID: 81897) in murine species. TLR9 is a single-pass type I membrane protein that serves as an innate immune sensor that recognizes microbial DNA. It exists as a monomer in the absence of unmethylated cytidine-phosphate-guanosine (CpG) ligand. TLR9 is synthesized with a signal peptide (aa 1-25), which is subsequently cleaved off to generate the mature form. The mature form contains an extracellular domain (aa 26-818), a short transmembrane domain (aa 819-839), and the cytoplasmic domain (aa 840-1032). It is expressed in the basolateral region of gastric epithelial cells and high levels are detected in antrum and body mucosa. Ligand recognition of TLR9 is shown to be strictly controlled to prevent unnecessary response to self-derived DNA such as mitochondrial DNA. Ligand-dependent TLR9 trafficking plays a critical role in type I interferon induction in macrophages and plasmacytoid dendritic cells. TLR9 is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides and acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion, and the inflammatory response. (Ref.: Murakami, Y., et al. (2017). Sci. Rep. 7; 44042).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in PBS without azide.
      Applications
      ApplicationAnti-TLR9, clone NaR9, Cat. No. MABF2156, is a mouse monoclonal antibody that detects Toll-like receptor 9 and has been tested for use in Flow Cytometry, Immunocytochemistry, and Inhibition/ Function Analysis.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Inhibits Activity/Function
      Application NotesImmunocytochemistry Analysis: A representative lot detected TLR9 in Immunocytochemistry applications (Murakami, Y., et. al. (2017). Sci Rep. 7:44042).

      Flow Cytometry Analysis: A representative lot detected TLR9 in Flow Cytometry applications (Murakami, Y., et. al. (2017). Sci Rep. 7:44042).

      Inhibits Activity/Function: A representative lot inhibited TLR9-dependent cytokine production in vitro by bone marrow-derived macrophages and conventional dendritic cells. (Murakami, Y., et. al. (2017). Sci Rep. 7:44042).
      Biological Information
      ImmunogenBa/F3 cells expressing full-length mouse TLR9 protein. Immunization was carried out on Tlr9 -/- mice.
      CloneNaR9
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone NaR9 is a mouse monoclonal antibody that specifically detects Toll-like receptor 9 in murine species. It targets an epitope within the N-terminal region.
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Tlr9
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight116.41 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in Raw264.7 cells.

      Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected TLR9 in Raw264.7 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      MABF2156-100UL 04061841030478

      Documentation

      Anti-TLR9 Antibody, clone NaR9 FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Anti-TLR9 Antibody, clone NaR9 Certificats d'analyse

      TitreNuméro de lot
      Anti-TLR9, clone NaR9 - 3756548 3756548
      Anti-TLR9, clone NaR9 - 4050739 4050739
      Anti-TLR9, clone NaR9 - 4113815 4113815
      Anti-TLR9, clone NaR9 - Q3269852 Q3269852

      Références bibliographiques

      Aperçu de la référence bibliographiqueNº PubMed
      The protective effect of the anti-Toll-like receptor 9 antibody against acute cytokine storm caused by immunostimulatory DNA.
      Murakami, Y; Fukui, R; Motoi, Y; Shibata, T; Saitoh, SI; Sato, R; Miyake, K
      Sci Rep  7  44042  2016

      Afficher le résumé       		28266597 28266597