Millipore Sigma Vibrant Logo

ABT257 Anti-Pro-Collagen Type I, A1/COL1A1

Anti-Pro-Collagen Type I Antibody is an antibody against Pro-Collagen Type I for use in Western Blotting, Flow Cytometry, Immunohistochemistry.

Anti-Pro-Collagen Type I, A1/COL1A1 : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: COL1A1 UniProt Number: P02452
View Products on Sigmaaldrich.com
ABT257
100 µL  
Prix en cours de récupération
Le prix n'a pas pu être récupéré
La quantité minimale doit être un multiple de
Maximum Quantity is
À la validation de la commande Plus d'informations
Vous avez sauvegardé ()
 
—
Demander le prix
—
Disponibilité limitée Disponibilité limitée
En stock 
Interrompu(e)
Disponible en quantités limitées
Disponibilité à confirmer
    Pour le restant : Nous vous tiendrons informé
      Pour le restant : Nous vous tiendrons informé
      Nous vous tiendrons informé
      Contacter le Service Clients
      Contact Customer Service

      Offres spéciales

       

      Contacter le Service Clients

      Aperçu

      Replacement Information

      Offres spéciales

      100% Performance Guaranteed

      Tableau de caractéristiques principal

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, FC, IHCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABT257
      DescriptionAnti-Pro-Collagen Type I, A1/COL1A1
      Alternate Names
      • Pro-collagen alpha-1(I) chain
      • Pro-Alpha-1 type I collagen
      Background InformationCollagen alpha-1(I) chain (UniProt P02452; also known as Alpha-1 type I collagen) is encoded by the COL1A1 gene (Gene ID 1277) in human. Collagen is the major component of the extracellular matrix (ECM) and form the fibrils of tendons, ligaments, and bones. Type I collagen consists of two alpha I chains and one alpha 2 chain. Alpha-1 type I collagen is initially produced as an 1464-amino acid prepro-form with a signal peptide sequence (a.a. 1-22) and two propeptide sequences (a.a. 23-161 and a.a. 1219 –1464), the removal of which yields the mature alpha-1(I) chain. The mature alpha-1(I) chain is composed mostly of a large triple-helical region (a.a. 179-1192) sandwiched between two nonhelical segments known as the N-terminal telopeptide (a.a. 162-178; numbering based on the prepro-form) and the C-terminal telopeptide (a.a. 1193-1218; numbering based on the prepro-form). Collagen can be extracted from tissue via either enzymatic or non-enzymatic means. Collagen extracted using the proteolytic enzyme pepsin corresponds to the large triple-helical region, referred to as atelocollagen because both the N- and C-terminal telopeptides have been cleaved off by pepsin. On the other hand, collagen preparations obtained with non-enzymatic means (e.g. by acid extraction) have the intact telopeptides at both ends.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Pro-Collagen Type I Antibody is an antibody against Pro-Collagen Type I for use in Western Blotting, Flow Cytometry, Immunohistochemistry.
      Key Applications
      • Western Blotting
      • Flow Cytometry
      • Immunohistochemistry
      Application NotesWestern Blotting Analysis: A representative lot detected Pro-Collagen Type I, A1/COL1A1 in mouse lung and skin tissue lysate.
      Flow Cytometry Analysis: A representative lot detected Pro-Collagen Type I, A1/COL1A1 in mouse lung tissue.
      Immunohistochemistry Analysis: A representative lot detected Pro-Collagen Type I, A1/COL1A1 in mouse lung tissue.
      Biological Information
      ImmunogenLinear peptide corresponding to sequence derived from human Pro-Collagen Type I, A1/COL1A1 C-terminal propeptide region.
      EpitopeC-terminal propeptide region
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityCat. No. ABT257 Anti-Pro-Collagen Type I, A1/COL1A1 was raised against a sequence derived from the C-terminal propeptide (not the N-terminal propeptide) region. This polyclonal antibody will detect prepro- and pro-, but not mature collagen alpha-1(I) chain.
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse. Predicted to react with a broad range of species based on high sequence homology, including mammalian, avian, fish, and amphibian species.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • COL1A1
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~140 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse skin tissue lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected Pro-Collagen Type I, A1/COL1A1 in 10 µg of mouse skin tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      ABT257 04055977136678

      Documentation

      Anti-Pro-Collagen Type I, A1/COL1A1 FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Anti-Pro-Collagen Type I, A1/COL1A1 Certificats d'analyse

      TitreNuméro de lot
      Anti-Pro-Collagen Type I, A1/COL1A1 2902699
      Anti-Pro-Collagen Type I, A1/COL1A1 - 3185049 3185049
      Anti-Pro-Collagen Type I, A1/COL1A1 - 3201707 3201707
      Anti-Pro-Collagen Type I, A1/COL1A1 - 3225879 3225879
      Anti-Pro-Collagen Type I, A1/COL1A1 - 3268885 3268885
      Anti-Pro-Collagen Type I, A1/COL1A1 - 3275493 3275493
      Anti-Pro-Collagen Type I, A1/COL1A1 - 3389910 3389910
      Anti-Pro-Collagen Type I, A1/COL1A1 - 3596724 3596724
      Anti-Pro-Collagen Type I, A1/COL1A1 - 3729946 3729946
      Anti-Pro-Collagen Type I, A1/COL1A1 - 3777980 3777980
      Show more

      Informations techniques

      Titre
      White Paper: Further considerations of antibody validation and usage.

      Produits & Applications associés

      Related Products

      Référence Description  
      AB745 Anti-Collagen Type I Antibody Prix & Disponibilité
      AB749P Anti-Collagen Type I Antibody Prix & Disponibilité
      AB752P Anti-Collagen Type I Antibody Prix & Disponibilité
      AB755P Anti-Rat Collagen Type I Antibody Prix & Disponibilité
      AB758 Anti-Collagen Type I Antibody Prix & Disponibilité
      AB758B Anti-Collagen Type I Antibody, biotin conjugated Prix & Disponibilité
      AB765P Anti-Mouse Collagen Type I Antibody Prix & Disponibilité
      ABT123 Anti-Collagen Type I Antibody Prix & Disponibilité
      ABT256 Anti-Telo-Collagen Type I, A1/COL1A1 Prix & Disponibilité
      FCMAB412F Milli-Mark® Anti-Collagen Type I-FITC Antibody, clone 5D8-G9 Prix & Disponibilité
      Show more

      Catégories

      Life Science Research > Antibodies and Assays > Primary Antibodies