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05-947 Anti-Phosphotyrosine Antibody, clone PY20

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05-947
1 mg  
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      Replacement Information
      Replacement Information05-947 is a recommended replacement for MAB3080

      Offres spéciales

      Tableau de caractéristiques principal

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      VrtELISA, IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-947
      ReplacesMAB3080
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Phosphotyrosine Antibody, clone PY20
      Background InformationTyrosine phosphorylation represents a major mechanism for the activation of signal transduction pathways in response to growth factors, hormones and cytokines. There are approximately 100 tyrosine kinases that are both receptor tyrsosine kinase receptors for various growth factors and cytokines. The extent and duration of tyrosine phosphorylation are tightly regulated by the 107 protein tyrosine phosphatases. Tyrosine phosphorylation occurs on residues on either other kinases (Ser/Thr or Tyr) or on substrates. In the case of kinases, this phosphorylation often causes the enzyme to change activation states and/or it creates binding sites for SH2 domain. Mutation or misregulation of protein tyrosine kinases is a cause or consequence of many disease states. It has been estimated that 20% of oncogenes so far described are tyrosine kinases. With such clear links between aberrant protein tyrosine kinase activity and disease, it is no surprise that much of the early effort in kinase drug discovery was focused on these enzymes.
      Product Information
      FormatPurified
      Control
      • EGF-stimulated A431 cell lysate.

        Included Positive Antigen Control: Catalog # 12-302, EGF-stimulated A431 cell lysate is provided as a free positive antigen control for western immunoblotting. Aliquot as desired, refreeze immediately, and store at -20°C. The lysate is stable for 6 months at -20°C. Before use, add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for immunoblot analysis.
      Presentation0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4. Liquid at 2-8°C.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Phosphotyrosine Antibody, clone PY20 is an antibody against Phosphotyrosine for use in ELISA, IP & WB.
      Key Applications
      • ELISA
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation:
      Recommended by an independent laboratory to immunoprecipitate tyrosine phosphorylated proteins.

      ELISA:
      Recommended by an independent laboratory.
      Biological Information
      ImmunogenPhosphotyrosine conjugated to carrier protein.
      ClonePY20
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityTyrosine-phosphorylated proteins from all species.
      IsotypeIgG2b
      Species Reactivity
      • Vertebrates
      Species Reactivity NoteMost common vertebrate species tested. Reactivity with other species has not been confirmed.
      Antibody TypeMonoclonal Antibody
      Modifications
      • Phosphorylation
      Purification MethodProtein A Purfied
      Molecular WeightDependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot with RIPA lysates EGF-treated human A431 carcinoma cells.

      Western Blot Analysis:
      0.5-2 μg/mL of this lot detected tyrosine-phosphorylated proteins in RIPA lysates EGFtreated human A431 carcinoma cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of recipt.

      NOTE: DO NOT FREEZE.
      For maximum recovery of the product, centrifuge the original vial prior to removing the cap. If the product has accidentally been frozen and thawed, spin it at 13,000 x g for 10 minutes at 2-8°C. Save the supernatant for application.
      Packaging Information
      Material Size1 mg
      Global Trade Item Number
      Référence GTIN
      05-947 04053252673641