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AB1987 Anti-Neurofilament M (145 kDa) Antibody, CT

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AB1987
50 µL  
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      Tableau de caractéristiques principal

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, Ch, H, M, Po, R, Qu, Rb, RpICC, IHC, IH(P), WBRbSerumPolyclonal Antibody
      Description
      Catalogue NumberAB1987
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Neurofilament M (145 kDa) Antibody, CT
      Background InformationNeurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments.
      Product Information
      FormatSerum
      Control
      • Rat enteric neurons, rat spinal cord tissue Brain, Peripheral Nerve
      PresentationNeat rabbit antiserum containing no preservative.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Neurofilament M (145 kDa) Antibody, C-terminus detects level of Neurofilament M (145 kDa) & has been published & validated for use in IC, IH, IH(P) & WB.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry:
      A 1:200 dilution of a previous lot was used in Immunohistochemistry.
      Staining is not affected by neurofilament phosphorylation. Optimal Staining With TE Buffer, pH 9.0, Epitope Retrieval: Human Cerebellum

      Immunohistochemistry(paraffin):

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenRecombinant fusion protein containing the C-terminal 168 amino acids of rat NF-M
      EpitopeC-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityStrong and specific staining for NF-M (145 kDa)
      Species Reactivity
      • Bovine
      • Chicken
      • Human
      • Mouse
      • Pig
      • Rat
      • Quail
      • Rabbit
      • Reptile
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NEFM
      • NF-M
      • NEF3
      • NFM
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P07197 # Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
      SIZE: 916 amino acids; 102448 Da
      PTM: There are a number of repeats of the tripeptide K-S-P, NFM is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFM results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. & Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
      SIMILARITY: SwissProt: P07197 ## Belongs to the intermediate filament family.
      Molecular Weight145 kDa
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on mouse brain lysates.

      Western Blot: 1:1000 dilution of this lot detected Neurofilament M on 10 μg of mouse brain lysates..
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC in undiluted aliquots from date of receipt.

      Handling Recommendations:
      Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size50 µL
      Global Trade Item Number
      Référence GTIN
      AB1987 04053252587696