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DR1050 Anti-NFE2L2 Mouse mAb (1F3)

This Anti-NFE2L2 Mouse mAb (1F3) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of NFE2L2.

Anti-NFE2L2 Mouse mAb (1F3) : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.

Synonymes: Anti-Nuclear Factor (Erythroid-Derived 2) Like 2

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      Recognizes the NFE2L2 protein in human lung tissue and HeLa cells.
      Catalogue NumberDR1050
      Brand Family Calbiochem®
      SynonymsAnti-Nuclear Factor (Erythroid-Derived 2) Like 2
      Application Data
      Detection of human NFE2L2 by immunoblotting. Sample: Partial recombinant NFE2L2. Primary antibody: Anti-NFE2L2 Mouse mAb (1F3) (Cat. No. DR1050) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human NFE2L2 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human lung tissue. Primary antibody: Anti-NFE2L2 Mouse mAb (1F3) (Cat. No. DR1050) (1 µg/ml). Detection: colorimetric (DAB).

      Detection of human NFE2L2 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-NFE2L2 Mouse mAb (1F3) (Cat. No. DR1050) (10 µg/ml). Detection: fluorescence.
      References
      ReferencesLindl, K,A., and Jordan-Sciutto, K,L., Methods Mol. Biol. 477 229.
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Positive controlHuman lung tissue, HeLa cells
      PreservativeNone
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Paraffin Sections
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Paraffin Sections (1 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids 71-170 of human NFE2L2, expressed as a GST fusion protein
      Clone1F3
      HostMouse
      IsotypeIgG2b
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      DR1050 0

      Documentation

      Anti-NFE2L2 Mouse mAb (1F3) FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Références bibliographiques

      Aperçu de la référence bibliographique
      Lindl, K,A., and Jordan-Sciutto, K,L., Methods Mol. Biol. 477 229.
      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision01-November-2010 RFH
      SynonymsAnti-Nuclear Factor (Erythroid-Derived 2) Like 2
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Paraffin Sections (1 µg/ml)
      Application Data
      Detection of human NFE2L2 by immunoblotting. Sample: Partial recombinant NFE2L2. Primary antibody: Anti-NFE2L2 Mouse mAb (1F3) (Cat. No. DR1050) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human NFE2L2 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human lung tissue. Primary antibody: Anti-NFE2L2 Mouse mAb (1F3) (Cat. No. DR1050) (1 µg/ml). Detection: colorimetric (DAB).

      Detection of human NFE2L2 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-NFE2L2 Mouse mAb (1F3) (Cat. No. DR1050) (10 µg/ml). Detection: fluorescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the NFE2L2 protein.
      BackgroundNFE2, NFE2L1, and NFE2L2 comprise a family of human genes encoding basic leucine zipper (bZIP) transcription factors. They share highly conserved regions that are distinct from other bZIP families, such as JUN and FOS, although remaining regions have diverged considerably from each other.
      HostMouse
      ImmunogenA recombinant polypeptide corresponding to amino acids 71-170 of human NFE2L2, expressed as a GST fusion protein
      Clone1F3
      IsotypeIgG2b
      Specieshuman
      Positive controlHuman lung tissue, HeLa cells
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review
      ReferencesLindl, K,A., and Jordan-Sciutto, K,L., Methods Mol. Biol. 477 229.