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NE1018 Anti-Myelin Basic Protein Mouse mAb (SMI-94)

This Anti-Myelin Basic Protein Mouse mAb (SMI-94) is validated for use in ELISA, Frozen Sections, Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of Myelin Basic Protein.

Anti-Myelin Basic Protein Mouse mAb (SMI-94) : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
NE1018
  
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      Aperçu

      Replacement Information

      Tableau de caractéristiques principal

      Species ReactivityHostAntibody Type
      Ca, H, Mk, M, Rb, RMMonoclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~21 kDa myelin basic protein in rat brain tissue.
      Catalogue NumberNE1018
      Brand Family Calbiochem®
      Application Data
      Detection of rat myelin basic protein by staining frozen sections. Sample: Rat brain. Primary antibody: Anti-Myelin Basic Protein Mouse mAb (SMI-94) (Cat. No. NE1018) (1:1000). Detection: fluorescence (green) with Hoechst counterstain.
      References
      ReferencesEvers, P. and Uylings, H.B. 1997. J. Neurosci. Methods 72, 197.
      Shin, R.W., et al. 1991. Lab. Invest. 64, 693.
      Product Information
      FormLiquid
      FormulationUndiluted ascites.
      Positive controlRat brain
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Frozen Sections
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Paraffin Sections
      Application NotesELISA (1:1000)
      Frozen Sections (1:1000, see comments)
      Immunoblotting (1:1000)
      Immunocytochemistry (1:1000)
      Paraffin Sections (1:1000, heat pre-treatment required, see comments)
      Application CommentsOther species have not been tested, but the antibody is expected to recognize MBP in most mammalian species. Recognizes developing and adult myelin and developing oligodendrocytes. Distinguishes oligodendrocytes from astrocytes, microglia, neurons, and other cells in brain tissue. In conjunction with Anti-Myelin Basic Protein Mouse mAb (SMI-91) (Cat. No. NE1020) and/or Anti-Myelin Basic Protein Mouse mAb (SMI-99) (Cat. No. NE1019), this antibody is useful for immunocytochemical studies on the progression of normal and pathologic myelination. Tissues can be fixed in a variety of paraformaldehyde- or formaldehyde-containing fixatives. To insure penetration of the antibody into myelin it is necessary to pre-treat frozen sections with cold methanol for 30 min or detergent. For staining paraffin sections it is recommended that de-paraffinized tissues be autoclaved in dH2O for 10 min or boiled in Tris-saline, pH 9.0, in a microwave for 15 min to expose the epitope. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenpurified human myelin basic protein
      ImmunogenHuman
      EpitopeWithin amino acids 70-89
      CloneSMI-94
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Canine
      • Human
      • Monkey
      • Mouse
      • Rabbit
      • Rat
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      NE1018 0

      Documentation

      Anti-Myelin Basic Protein Mouse mAb (SMI-94) FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Anti-Myelin Basic Protein Mouse mAb (SMI-94) Certificats d'analyse

      TitreNuméro de lot
      NE1018

      Références bibliographiques

      Aperçu de la référence bibliographique
      Evers, P. and Uylings, H.B. 1997. J. Neurosci. Methods 72, 197.
      Shin, R.W., et al. 1991. Lab. Invest. 64, 693.
      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision01-October-2007 RFH
      ApplicationELISA (1:1000)
      Frozen Sections (1:1000, see comments)
      Immunoblotting (1:1000)
      Immunocytochemistry (1:1000)
      Paraffin Sections (1:1000, heat pre-treatment required, see comments)
      Application Data
      Detection of rat myelin basic protein by staining frozen sections. Sample: Rat brain. Primary antibody: Anti-Myelin Basic Protein Mouse mAb (SMI-94) (Cat. No. NE1018) (1:1000). Detection: fluorescence (green) with Hoechst counterstain.
      DescriptionMouse monoclonal antibody supplied as undiluted ascites. Recognizes the ~21 kDa myelin basic protein (MBP).
      BackgroundThe myelin sheath is essential for rapid and effective propagation of action potential within the axon. Mutations identified in several myelin-related genes reportedly lead to severe demyelinating neuropathies. Recent evidence also suggests that the autoimmune disease Multiple Sclerosis (MS) is mediated by T cells with specificity for antigens in the myelin sheath. Although myelin in the central nervous system (CNS) and peripheral nervous system (PNS) is structurally similar, it is chemically distinct since oligodendrocytes form myelin in the CNS whereas Schwann cells generate PNS myelin. Myelin proteolipid protein (PLP) is the predominant integral membrane protein in mammalian CNS myelin. In contrast, myelin basic protein (MBP) is localized to the myelin cytoplasm and is highly conserved among species. Both MBP and PLP mediate myelin compaction, whereas thickness is regulated by MBP. Several other myelin proteins have been identified to play distinct or partially overlapping functions in the formation and maintenance of the myelin sheath, including P0, myelin-associated glycoprotein (MAG), peripheral myelin protein (PMP22), and Connexin 32 (Cx32). The development of the myelin sheath has been a good model for understanding the mechanisms of neuron-glia interaction.
      HostMouse
      Immunogen speciesHuman
      Immunogenpurified human myelin basic protein
      EpitopeWithin amino acids 70-89
      CloneSMI-94
      IsotypeIgG₁
      Speciescanine, human, monkey, mouse, rabbit, rat
      Positive controlRat brain
      FormLiquid
      FormulationUndiluted ascites.
      Preservative≤0.1% sodium azide
      CommentsOther species have not been tested, but the antibody is expected to recognize MBP in most mammalian species. Recognizes developing and adult myelin and developing oligodendrocytes. Distinguishes oligodendrocytes from astrocytes, microglia, neurons, and other cells in brain tissue. In conjunction with Anti-Myelin Basic Protein Mouse mAb (SMI-91) (Cat. No. NE1020) and/or Anti-Myelin Basic Protein Mouse mAb (SMI-99) (Cat. No. NE1019), this antibody is useful for immunocytochemical studies on the progression of normal and pathologic myelination. Tissues can be fixed in a variety of paraformaldehyde- or formaldehyde-containing fixatives. To insure penetration of the antibody into myelin it is necessary to pre-treat frozen sections with cold methanol for 30 min or detergent. For staining paraffin sections it is recommended that de-paraffinized tissues be autoclaved in dH2O for 10 min or boiled in Tris-saline, pH 9.0, in a microwave for 15 min to expose the epitope. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesEvers, P. and Uylings, H.B. 1997. J. Neurosci. Methods 72, 197.
      Shin, R.W., et al. 1991. Lab. Invest. 64, 693.