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475923 Anti-Muscarinic M1 Acetylcholine Receptor (227-353) Rabbit pAb

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Synonymes: Anti-M1

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      Aperçu

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      This product has been discontinued.





      Recognizes the ~120 kDa muscarinic M1 acetylcholine receptor protein in rat brain. Supplied with a control peptide.
      Catalogue Number475923
      Brand Family Calbiochem®
      SynonymsAnti-M1
      Application Data
      Detection of rat muscarinic M1 acetylcholine receptor by immunoblotting. Samples: Extract from rat brain membranes left untreated (lane 1) and treated by preincubation with the fusion protein antigen (lane 2). Primary antibody: Anti-Muscarinic M1 Acetylcholine Receptor (227-353) Rabbit pAb (Cat. No. 475923) (1:200). Detection: chemiluminescence.
      References
      ReferencesLevey, A.I., et al. 1991. J. Neurosci. 11, 3218.
      Levey, A.I., et al. 1990. FEBS Lett. 275, 65.
      Smith. D.B., et al. 1988. Gene 67, 31.
      Allard, W.J., et al. 1987. Nucleic Acids Res. 15, 10604.
      Peralta, E.G., et al. 1987. EMBO J. 6, 3923.
      Product Information
      FormLyophilized
      Formulation50 µl antibody lyophilized from PBS, 1% BSA, pH 7.4 and 60 µg lyophilized control protein.
      Positive controlM1-GST fusion protein (supplied) or rat brain
      Preservative≤0.1% sodium azide (antibody only)
      Applications
      Application ReferencesImmunohistochemistry, Immunoprecipitation Levey, A.I., et al. 1991. J. Neurosci. 11, 3218. Immunoprecipitation Levey, A.I., et al. 1990. FEBS Lett. 275, 65.
      Key Applications Frozen Sections
      Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesImmunoblotting (1:200-1:300, chemiluminescence)
      Immunohistochemistry (see application references)
      Immunoprecipitation (see application references)
      Application CommentsThe epitope is specific for M1 and is not similar to any other known protein. To reduce non-specific staining, centrifuge all antibody preparations before use (10000 x g for 5 min) to remove any aggregates. For use as a positive control in immunoblotting, use 10 ng/lane of the M1-GST fusion protein (minigel). For use as a negative control, preincubate 3-5 µg of M1-GST fusion protein with 1 µg antibody for 1 h at room temperature. Variables associated with assay conditions will dictate the optimal working dilution.
      Biological Information
      Immunogena recombinant protein consisting of amino acids 227-353 of the i3 intracellular loop of human M1 muscarinic acetylcholine receptor fused to GST
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute the antibody with 50 µl dH₂O to yield a stock concentration of 300 µg/ml. For long-term storage, aliquot the antibody and freeze (-20°); for short-term storage, refrigerate (4°C). Further dilutions should be made in a physiological buffer containing a carrier protein such as 1-3% BSA. Reconstitute the M1-GST fusion protein with 100 µl H₂O, aliquot, and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      475923 0

      Documentation

      Références bibliographiques

      Aperçu de la référence bibliographique
      Levey, A.I., et al. 1991. J. Neurosci. 11, 3218.
      Levey, A.I., et al. 1990. FEBS Lett. 275, 65.
      Smith. D.B., et al. 1988. Gene 67, 31.
      Allard, W.J., et al. 1987. Nucleic Acids Res. 15, 10604.
      Peralta, E.G., et al. 1987. EMBO J. 6, 3923.
      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision07-August-2007 RFH
      SynonymsAnti-M1
      ApplicationImmunoblotting (1:200-1:300, chemiluminescence)
      Immunohistochemistry (see application references)
      Immunoprecipitation (see application references)
      Application Data
      Detection of rat muscarinic M1 acetylcholine receptor by immunoblotting. Samples: Extract from rat brain membranes left untreated (lane 1) and treated by preincubation with the fusion protein antigen (lane 2). Primary antibody: Anti-Muscarinic M1 Acetylcholine Receptor (227-353) Rabbit pAb (Cat. No. 475923) (1:200). Detection: chemiluminescence.
      DescriptionRabbit polyclonal antibody adsorbed against immobilized non-relevant GST fusion protein followed by immunoaffinity chromatography. Recognizes the ~120 kDa muscarinic M1 acetylcholine receptor protein. Supplied with a control protein consisting of recombinant M1-GST fusion protein.
      HostRabbit
      Immunogen speciesHuman
      Immunogena recombinant protein consisting of amino acids 227-353 of the i3 intracellular loop of human M1 muscarinic acetylcholine receptor fused to GST
      IsotypeIgG
      Specieshuman, rat
      Positive controlM1-GST fusion protein (supplied) or rat brain
      FormLyophilized
      Formulation50 µl antibody lyophilized from PBS, 1% BSA, pH 7.4 and 60 µg lyophilized control protein.
      Preservative≤0.1% sodium azide (antibody only)
      CommentsThe epitope is specific for M1 and is not similar to any other known protein. To reduce non-specific staining, centrifuge all antibody preparations before use (10000 x g for 5 min) to remove any aggregates. For use as a positive control in immunoblotting, use 10 ng/lane of the M1-GST fusion protein (minigel). For use as a negative control, preincubate 3-5 µg of M1-GST fusion protein with 1 µg antibody for 1 h at room temperature. Variables associated with assay conditions will dictate the optimal working dilution.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute the antibody with 50 µl dH₂O to yield a stock concentration of 300 µg/ml. For long-term storage, aliquot the antibody and freeze (-20°); for short-term storage, refrigerate (4°C). Further dilutions should be made in a physiological buffer containing a carrier protein such as 1-3% BSA. Reconstitute the M1-GST fusion protein with 100 µl H₂O, aliquot, and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
      Toxicity Standard Handling
      ReferencesLevey, A.I., et al. 1991. J. Neurosci. 11, 3218.
      Levey, A.I., et al. 1990. FEBS Lett. 275, 65.
      Smith. D.B., et al. 1988. Gene 67, 31.
      Allard, W.J., et al. 1987. Nucleic Acids Res. 15, 10604.
      Peralta, E.G., et al. 1987. EMBO J. 6, 3923.
      Application referencesImmunohistochemistry, Immunoprecipitation Levey, A.I., et al. 1991. J. Neurosci. 11, 3218. Immunoprecipitation Levey, A.I., et al. 1990. FEBS Lett. 275, 65.