Millipore Sigma Vibrant Logo

MAB367 Anti-Muscarinic Acetylcholine Receptor m2 Antibody, clone M2-2-B3

View Products on Sigmaaldrich.com
MAB367
100 µg  
Le prix n'a pas pu être récupéré
La quantité minimale doit être un multiple de
Maximum Quantity is
À la validation de la commande Plus d'informations
Vous avez sauvegardé ()
 
Demander le prix
Disponibilité limitée
Disponibilité limitée
En stock 
Interrompu(e)
Disponible en quantités limitées
Disponibilité à confirmer
    Pour le restant : Nous vous tiendrons informé
      Pour le restant : Nous vous tiendrons informé
      Nous vous tiendrons informé
      Contacter le Service Clients
      Contact Customer Service

      Offres spéciales

       

      Contacter le Service Clients

      Aperçu

      Offres spéciales

      Tableau de caractéristiques principal

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Ma, Mk, RICC, IHC, IPRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB367
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Muscarinic Acetylcholine Receptor m2 Antibody, clone M2-2-B3
      Product Information
      FormatPurified
      PresentationPurified immunoglobulin. Liquid in 0.02 M phosphate buffer, 0.25 M NaCl with 0.1% sodium azide, pH 7.6.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Muscarinic Acetylcholine Receptor m2 Antibody, clone M2-2-B3 is an antibody against Muscarinic Acetylcholine Receptor m2 for use in IC, IH & IP.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunoprecipitation
      Applications Not Recommended
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry on 4% paraformaldehyde fixed tissue. Does not work on paraffin embedded tissues. Suggested starting concentration 1-5 μg/mL. It is suggested that you use the PAP system if using this antibody on rat. Immunocytochemistry on transfected cells

      Immunoprecipitation Works poorly for immunoblotting Optimal working dilutions must be determined by end user.

      IMMUNOHISTOCHEMISTRY PROTOCOL FOR MAB367

      This antibody has been used successfully on 30 mm, free floating, 4% paraformaldehyde fixed rat brain tissue. All steps are performed under constant agitation. Suggested protocol follows.

      1) 3 x 10 minute washes in TBS (with or without 0.25% Triton).

      2) Incubate for 30 minutes in TBS with 3% serum (same as host from secondary antibody).

      3) Incubate primary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody) (with or without 0.25% Triton) for 2 hours at room temperature followed by 16 hours at 4°C.

      4) 3 x 10 minute washes in TBS.

      5) Incubate with secondary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody).

      6) 3 x 10 minute washes in TBS.

      7) ABC Elite (1:200 Vector Labs) in TBS.

      8) 2 x 10 minute washes in TBS.

      9) 1 x 10 minute wash in phosphate buffer (no saline).

      10) DAB reaction with 0.06% NiCl added for intensification.

      11) 2 x 10 minute washes in PBS.

      12) 1 x 10 minute wash in phosphate buffer (no saline).
      Biological Information
      Immunogeni3 loop of m2 receptor fusion protein (225-359), fused to Glutathione S-transferase.
      CloneM2-2-B3
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRat
      Specificitym2 muscarinic acetylcholine receptor. No reactivity with the other subtypes.

      SPECIES REACTIVITIES:

      Expected to react with most mammalian species.
      IsotypeIgG2a
      Species Reactivity
      • Human
      • Mammals
      • Monkey
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine to these receptors and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 2 is involved in mediation of bradycardia and a decrease in cardiac contractility. Multiple alternatively spliced transcript variants have been described for this gene.
      Gene Symbol
      • CHRM2
      • MGC120006
      • FLJ43243
      • MGC120007
      • HM2
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P08172 # The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition.
      SIZE: 466 amino acids; 51715 Da
      SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
      SIMILARITY: SwissProt: P08172 ## Belongs to the G-protein coupled receptor 1 family.
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C in undiluted aliquots for up to 6 months.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Référence GTIN
      MAB367 04053252660566