Millipore Sigma Vibrant Logo

AB8345 Anti-MMP-14 Antibody

View Products on Sigmaaldrich.com
AB8345
100 µL  
Le prix n'a pas pu être récupéré
La quantité minimale doit être un multiple de
Maximum Quantity is
À la validation de la commande Plus d'informations
Vous avez sauvegardé ()
 
Demander le prix
Disponibilité limitée
Disponibilité limitée
En stock 
Interrompu(e)
Disponible en quantités limitées
Disponibilité à confirmer
    Pour le restant : Nous vous tiendrons informé
      Pour le restant : Nous vous tiendrons informé
      Nous vous tiendrons informé
      Contacter le Service Clients
      Contact Customer Service

      Offres spéciales

       

      Contacter le Service Clients

      Aperçu

      Offres spéciales

      Tableau de caractéristiques principal

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HELISA, FC, IP, WB, ICCRbSerumPolyclonal Antibody
      Description
      Catalogue NumberAB8345
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-MMP-14 Antibody
      Alternate Names
      • MT1-MMP
      Product Information
      FormatSerum
      Control
      • Immunocytochemistry positive control: U251 glioma cells; negative control MCF7 breast carcinoma.

        Western Blot positive control: HT1080 fibrosarcoma, negative control: MCF7 breast carcinoma
      PresentationLiquid in PBS, pH 7.6, containing 0.01% sodium azide as a preservative.
      Quality LevelMQ100
      Applications
      ApplicationAnti-MMP-14 Antibody detects level of MMP-14 & has been published & validated for use in ELISA, FC, IP, WB & IC.
      Key Applications
      • ELISA
      • Flow Cytometry
      • Immunoprecipitation
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry: 5-10 μg / ml

      Western blot: 5 μg / ml Expected band sizes: 60-63 kDA and 42 kDa pro-enzyme and degradation form respectively.

      Immunopreciptation: 1-2 μg / mg total protein in 500 μl reaction volume.

      FACS Analysis: 5 μg/ mL (1 million cells per mL)

      EIA: 1:10,000

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenCatalytic domain of MT1-MMP
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThe antibody recognizes native and recombinant proteins.
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryProteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion.
      Gene Symbol
      • MMP14
      • MTMMP1
      • MMP-14
      • MT1MMP
      • MT1-MMP
      • MMP-X1
      • EC 3.4.24.80
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P50281 # Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface.
      COFACTOR: Binds 1 zinc ion per subunit (By similarity). & Calcium (By similarity).
      SIZE: 582 amino acids; 65884 Da
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein (Potential). Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
      TISSUE SPECIFICITY: In stromal cells of colon, breast, and head and neck.
      DOMAIN: SwissProt: P50281 The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
      SIMILARITY: Belongs to the peptidase M10A family. & Contains 4 hemopexin-like domains.
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore antibody at -20°C for 12 months. Avoid repeat freeze thaw cycles.
      Packaging Information
      Material Size100 µL
      Global Trade Item Number
      Référence GTIN
      AB8345 04053252282942