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MABF2257-25UG Anti-HHV-8 ORF45 Antibody, clone ORF45

Anti-HHV-8 ORF45, clone ORF45, Cat. No. MABF2257, is a mouse monoclonal antibody that detects ORF45 in HHV-8 (Kaposi's sarcoma-associated herpesvirus) and is tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

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MABF2257-25UG
25 μg  
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      Replacement Information
      Description
      Catalogue NumberMABF2257-25UG
      DescriptionAnti-HHV-8 ORF45 Antibody, clone ORF45
      Alternate Names
      • ORF45
      • KSHV ORF45
      Background InformationHuman herpesvirus-8 (also known as Kaposi's sarcoma-associated herpesvirus, HHV-8, KSHV) is etiologically associated with all forms of Kaposi sarcoma (KS) including classic, endemic, transplant-related, and AIDS-related, as well as rare neoplastic disorders. It is a double stranded DNA virus with a large genome that encodes for over 87 open reading frames (ORFs) including genes necessary for capsid, tegument, envelope, DNA replication and regulatory proteins. HHV-8 undergoes a bi-phasic lifecycle and can persist indefinitely in the infected host in a latent form during which time only a small fraction of regulatory viral proteins is expressed. ORF45 is an immediate-early phosphoprotein that localizes in the cytoplasm and acts as a viral lytic protein. It interacts with KIF3A, a subunit of kinesin-2 motor protein that transports cargoes along microtubules to cell periphery. The N-terminal amino acids 1-115 and C-terminal amino acids 332-407 of ORF45 are reported to interact with KIF3A. Inhibition of KIF3A-ORF45 interaction is shown to reduce HHV-8 maturation and egress. ORF45 also interacts with interferon regulatory factor 7 and inhibits virus-mediated interferon a/b induction. (Ref.: Sathish, N., et al. (2009). PLoS Pathog 5(3): e1000332; Zhu, FX., et al. (2005). J. Virol. 79(2);800-811; Zhu, FX., and Yuan, Y. (2003). J. Virol. 77(7); 4221-4230).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-HHV-8 ORF45, clone ORF45, Cat. No. MABF2257, is a mouse monoclonal antibody that detects ORF45 in HHV-8 (Kaposi's sarcoma-associated herpesvirus) and is tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunocytochemistry
      Application NotesTested Applications

      Western Blotting Analysis: A representative lot detected HHV-8 ORF45 in Western Blotting applications (Zhu, F.X., et al. (2005). J Virol. 79(2):800-11; Sathish, N., et al. (2009). PLoS Pathog. 5(3):e1000332; De Leo, A., et al. (2017). PLoS Pathog. 13(8):e1006596).

      Immunoprecipitation Analysis: A representative lot detected HHV-8 ORF45 in Immunoprecipitation applications (Sathish, N., et al. (2009). PLoS Pathog. 5(3):e1000332).

      Immunocytochemistry Analysis: A representative lot detected HHV-8 ORF45 in Immunocytochemistry applications (Sathish, N., et al. (2009). PLoS Pathog. 5(3):e1000332).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenBaculovirus-synthesized ORF45 protein.
      EpitopeUnknown
      CloneORF45
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone ORF45 is a mouse monoclonal antibody that detects ORF45 protein in HHV-8 (Kaposi's sarcoma-associated herpesvirus).
      IsotypeIgG1κ
      Species Reactivity
      • Virus
      Species Reactivity NoteKaposi's sarcoma-associated herpesvirus (HHV-8).
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • ORF45
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~70 kDa observed; 43.33 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in TPA-induced BCBL-1 cell lysates.

      Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected HHV-8 (Kaposi's sarcoma-associated herpesvirus) ORF45 protein in lysates from 12-O-Tetradecanoylphorbol-13-acetate (TPA; 20 ng/mL; 5-7 days)-induced BCBL-1 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      MABF2257-25UG 04065267061566