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AB3080P Anti-Green Fluorescent Protein Antibody

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AB3080P
50 µg  
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      Tableau de caractéristiques principal

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Vrt, RELISA, ICC, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB3080P
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Green Fluorescent Protein Antibody
      Alternate Names
      • GFP
      • eGFP
      Background InformationThe Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27 kDa monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.
      Product Information
      FormatAffinity Purified
      Control
      • Cells transfected with GFP-tagged fusion vector
      • RENCX cells
      PresentationAffinity purified rabbit polyclonal IgG in 10 mM Tris buffer.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Green Fluorescent Protein Antibody detects level of Green Fluorescent Protein & has been published & validated for use in ELISA, IC & WB.
      Key Applications
      • ELISA
      • Immunocytochemistry
      • Western Blotting
      Application NotesWestern Blot:
      A 1:500-1:1000 dilution of a previous lot was used using colorimetric detection (Peroxidase/TMB), with higher dilution possible using more sensitive detection methods.

      ELISA:
      A 1:800-1:1,000 dilution of a previous lot was used in ELISA.

      Note: While AB3080 has been adsorbed against E. coli proteins, most secondary antibodies have not. To reduce the likelihood of E. coli reactivity from a secondary antibody, we suggest the use of Millipore's highly purified secondary antibodies in indirect detection systems.

      Immunocytochemistry:

      Optimal working dilutions and protocols must be determined by end user.
      Biological Information
      ImmunogenHighly purified native GFP from Aequorea victoria.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityAB3080P is made against highly-purified native GFP from Aequorea victoria. It is reactive with GFP from both native and recombinant sources. While other commercial antibodies to GFP typically demonstrate strong reactivity with E. coli, the AB3080P GFP antibody has been affinity purified, followed by extensive adsorption against whole E. coli BL21 strain cells, giving them minimal cross-reactivity with E. coli.
      Species Reactivity
      • Vertebrates
      • Rat
      Species Reactivity NoteMost common vertebrate species tested. Reactivity with other species has not been confirmed. Expected to react with rat based on sequence homology.
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • GFP
      • eGFP
      Purification MethodAffinity purified rabbit immunoglobulin
      UniProt Number
      Molecular WeightVaries (GFP is 27 kDa)
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunocytochemistry on RENCX cells.

      Immunocytochemistry Analysis: Confocal fluorescent analysis of RENCX constitutively expressed GFP using this lot's anti-GFP Rabbit pAB (Red). Green indicates the expressed GFP.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C in undiluted aliquots from date of receipt.

      Handling Recommendations:
      Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
      Packaging Information
      Material Size50 µg
      Global Trade Item Number
      Référence GTIN
      AB3080P 04053252269547