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AB10529 Anti-GluR-2 Antibody, CT

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AB10529
100 µL  
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      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, MWBRbSerumPolyclonal Antibody
      Description
      Catalogue NumberAB10529
      DescriptionAnti-GluR-2 Antibody, CT
      Alternate Names
      • glutamate receptor, ionotropic, AMPA 2
      • glutamate receptor 2
      • Glutamate receptor ionotropic, AMPA 2
      • AMPA-selective glutamate receptor 2
      Background InformationGlutamate receptors (GluRs) can be categorized as ionotropic or metabotropic and subcatergorized by their agonist preferences (NMDA, AMPA or Kainic acid). There are four types of AMPA selective GluR subunits (GluR1, GluR2, GluR3 and GluR4). Tetrameric or pentameric combinations of different subunits contributes to the functional diversity of AMPA receptors. In general, AMPA receptors mediate fast synaptic current at most excitatory synapses, with stoichiometry characterized by subtype composition. Although subunit composition of AMPA receptors varies, they must contain at least one edited GluR2 subunit to be calcium impermeable. The critical residue controlling calcium permeability is in the pore loop region. In GluR1, GluR3, and GluR4, this positionis occupied by a Gln residue. In GluR2, it is occupied by an Arg residue. It has been shown experimentally that the presence of Arg in this position blocks CA2+ ion permeability, while a Gln does not. Relative calcium permeability in AMPA receptor channels may be significant in pathological neurotoxic damage and long term changes in nervous system responses.
      References
      Product Information
      FormatSerum
      Control
      • PC12 cell lysate
      PresentationRabbit polyclonal serum containing 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-GluR-2 Antibody, C-terminus detects level of GluR-2 & has been published & validated for use in WB.
      Key Applications
      • Western Blotting
      Application NotesImmunoprecipitation: A previous lot of this antibody successfully immoprecipitated GluR-2 from a brain tissue lysate, as reported by an independent laboratory.
      Biological Information
      ImmunogenRecombinant protein corresponding to the C-terminus of rat GluR-2.
      EpitopeC-terminus
      HostRabbit
      SpecificityThis antibody recognizes GluR-2 at the C-terminus.
      Species Reactivity
      • Rat
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryGlutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels. These channels are assembled from 4 related subunits, Gria1-4. The subunit encoded by this gene (Gria2) is subject to RNA editing (Q/R and R/G), which is thought to render the channels impermeable to Ca(2+), and to affect the kinetic aspects of these channels in rat brain. Alternative splicing, resulting in transcript variants encoding different isoforms (flip and flop), has been noted for this gene. [provided by RefSeq].
      Gene Symbol
      • HBGR2
      • GLUR2
      • GLURB
      • GluR-K2
      • GluR-B
      • GluR-2
      • gluR-B
      • GRIA2
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.

      SIZE: 883 amino acids; 98,688 Da

      SUBUNIT: Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers. May interact with MPP4. Forms a ternary complex with GRIP1 and CSPG4(By similarity). Interacts with CACNG2, PRKCABP, GRIP1 and GRIP2. Interacts with NSF via its C-terminus. Part of a complex containing GRIA2, NSF and NAPA and/or NAPB.

      SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Note= Interaction with CACNG2 promotes cell surface expression.

      TISSUE SPECIFICITY: Detected in forebrain. Detected in dendrites of neuronal cells.

      SIMILARITY: Belongs to the glutamate-gated ion channel (TC 1.A.10) family.
      Molecular Weight ~106 kDa Observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in PC12 cell lysate.

      Western Blot Analysis: A 1:1,000 dilution of this antibody detected GluR-2 on 10 µg of PC12 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      AB10529 04053252518591

      Documentation

      Anti-GluR-2 Antibody, CT FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Anti-GluR-2 Antibody, CT Certificats d'analyse

      TitreNuméro de lot
      Anti-GluR-2, C-terminus 2475901
      Anti-GluR-2, C-terminus - 2127822 2127822
      Anti-GluR-2, C-terminus - 2441974 2441974
      Anti-GluR-2, C-terminus - 1990711 1990711
      Anti-GluR-2, C-terminus - 2033664 2033664
      Anti-GluR-2, C-terminus - 2067249 2067249
      Anti-GluR-2, C-terminus - 2276394 2276394
      Anti-GluR-2, C-terminus - 2303968 2303968
      Anti-GluR-2, C-terminus - 3245148 3245148
      Anti-GluR-2, C-terminus - 4006261 4006261

      Références bibliographiques

      Aperçu de la référence bibliographiqueApplicationNº PubMed
      Nerve injury increases GluA2-lacking AMPA receptor prevalence in spinal cords: functional significance and signaling mechanisms.
      Chen, SR; Zhou, HY; Byun, HS; Pan, HL
      The Journal of pharmacology and experimental therapeutics  347  765-72  2013

      Afficher le résumé
      Western Blotting24030012 24030012
      Reactive oxygen species (ROS) modulate AMPA receptor phosphorylation and cell-surface localization in concert with pain-related behavior.
      Lee, DZ; Chung, JM; Chung, K; Kang, MG
      Pain  153  1905-15  2011

      Afficher le résumé
      22770842 22770842

      Brochure

      Titre
      Pathways and Biomarkers of Glutamatergic Synapse Flyer

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      Catégories

      Life Science Research > Antibodies and Assays > Primary Antibodies