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PC213L Anti-GABA Rabbit pAb

Anti-GABA, rabbit polyclonal, recognizes GABA in rat thalamus and cerebellum. It is validated for use in immunohistochemistry on frozen sections.

Anti-GABA Rabbit pAb : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.

Synonymes: Anti-Gamma Amino-Butyric Acid

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PC213L
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Replacement Information

Tableau de caractéristiques principal

Species ReactivityHostAntibody Type
RRbPolyclonal Antibody

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PC213L-100UL
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      Description
      OverviewRecognizes GABA in rat thalamus and cerebellum.
      Catalogue NumberPC213L
      Brand Family Calbiochem®
      SynonymsAnti-Gamma Amino-Butyric Acid
      Application Data
      Detection of rat GABA by immunohistochemistry. Sample: Rat thalamus and cerebellum. Primary antibody: Anti-GABA Rabbit pAb (Cat. No. PC213L) (1:20,000). Detection: fluorescence.
      References
      ReferencesSudhof, T.C. 1995. Nature 375, 645.
      Hokfelt, T., et al. 1987. Experientia Suppl. 56, 154.
      Product Information
      FormLyophilized
      FormulationUndiluted serum.
      Positive controlRat thalamus or cerebellum
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Frozen Sections
      Application NotesFrozen Sections (1:12,000-1:15,000, ABC detection or 1:3000-1:5000, PAP detection; see comments)
      Application CommentsAntibody specificity was examined in the rat thalamus and cerebellum. Glutaraldehyde is a necessary component of fixation for use with this antibody. Higher concentrations of glutaraldehyde (1%-2%) may be used as needed. The specificity of the antibody for GABA was evaluated using a competitive inhibition ELISA. A GABA conjugate control completely eliminated labeling, while 1000-fold excess of the following conjugates: alanine, aspartate, beta alanine, glutamate, glycine, taurine, and tyrosine did not inhibit the binding to the GABA conjugate control. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      ImmunogenGABA
      ImmunogenRat
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Rat
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. For storage, dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      PC213L-100UL 04055977224665

      Documentation

      Anti-GABA Rabbit pAb FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Anti-GABA Rabbit pAb Certificats d'analyse

      TitreNuméro de lot
      PC213L

      Références bibliographiques

      Aperçu de la référence bibliographique
      Sudhof, T.C. 1995. Nature 375, 645.
      Hokfelt, T., et al. 1987. Experientia Suppl. 56, 154.
      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision09-October-2007 RFH
      SynonymsAnti-Gamma Amino-Butyric Acid
      ApplicationFrozen Sections (1:12,000-1:15,000, ABC detection or 1:3000-1:5000, PAP detection; see comments)
      Application Data
      Detection of rat GABA by immunohistochemistry. Sample: Rat thalamus and cerebellum. Primary antibody: Anti-GABA Rabbit pAb (Cat. No. PC213L) (1:20,000). Detection: fluorescence.
      DescriptionRabbit polyclonal antibody supplied as lyophilized undiluted serum. Recognizes GABA.
      BackgroundNeurotransmitters can be defined as chemical substances that are released by the presynaptic neuron before being bound by receptors on the postsynaptic cell surface. It has been well established that the nervous system utilizes neurotransmitters for cell signaling. Neurotransmitters can be divided into two groups; low molecular weight transmitter molecules such as biogenic amines, purines, or amino acids, and bioactive peptides, of which more than 50 members have been identified in the mammalian central nervous system. Many low molecular weight neurotransmitters are produced within the axon terminal from common cellular metabolites and packaged into synaptic vesicles. In contrast, neuropeptides are synthesized in the cell body and packaged into large dense core vesicles for delivery to the presynaptic membrane. The transport and delivery of vesicles containing neurotransmitter is a highly regulated and complex process. GABA (g-aminobutyric acid) is the major inhibitory neurotransmitter in the central nervous system that is involved in the pathogenesis of certain neurological and psychiatric disorders. GABA is produced from glutamic acid in a reaction catalyzed by glutamic acid decarboxylase. GABA interacts with the GABAA and GABAB receptors, which are widely distributed throughout the nervous system and in a variety of cell types. The immunolocalization, neuronal distribution, and vesicular compartmentalization of neurotransmitter molecules and their precursors are important for understanding the modulation, regulation, and function of neurotransmitters in the nervous system.
      HostRabbit
      Immunogen speciesRat
      ImmunogenGABA
      IsotypeIgG
      Speciesrat
      Positive controlRat thalamus or cerebellum
      FormLyophilized
      FormulationUndiluted serum.
      PreservativeNone
      CommentsAntibody specificity was examined in the rat thalamus and cerebellum. Glutaraldehyde is a necessary component of fixation for use with this antibody. Higher concentrations of glutaraldehyde (1%-2%) may be used as needed. The specificity of the antibody for GABA was evaluated using a competitive inhibition ELISA. A GABA conjugate control completely eliminated labeling, while 1000-fold excess of the following conjugates: alanine, aspartate, beta alanine, glutamate, glycine, taurine, and tyrosine did not inhibit the binding to the GABA conjugate control. Antibody should be titrated for optimal results in individual systems.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. For storage, dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.
      Toxicity Standard Handling
      ReferencesSudhof, T.C. 1995. Nature 375, 645.
      Hokfelt, T., et al. 1987. Experientia Suppl. 56, 154.