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GR40 Anti-Estrogen Receptor β (Ab-3) Mouse mAb (10.76)

FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.

Synonymes: Anti-ER β

GR40
  
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      Aperçu

      Replacement Information

      Tableau de caractéristiques principal

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      Description
      Overview

      This product has been discontinued.





      Recognizes the ~53 kDa estrogen receptor β protein. Does not cross-react with estrogen receptor α.
      Catalogue NumberGR40
      Brand Family Calbiochem®
      SynonymsAnti-ER β
      Application Data
      Detection of human estrogen receptor β by immunoprecipitation followed by immunoblotting. Samples: Recombinant estrogen receptor β (5 μg). Immunoprecipitating antibody: Anti-Estrogen Receptor β (Ab-3) Mouse mAb (10.76) (Cat. No. GR40) (1 μg). Conditions for immunoblotting: Samples: Recombinant estrogen receptor β (200 ng, unprecipitated) (lane A) and recombinant estrogen receptor β (immunoprecipitated above, lane B). Primary antibody: Anti-Estrogen Receptor β (Ab-2) Mouse mAb (9.88) (Cat. No. GR39) (1:1000). Detection: chemiluminescence.
      References
      ReferencesShughrue, P.J., et al. 1998. Endocrinol. 139, 5267.
      Al-Shamma, H.A. and Arnold, A.P. 1997. Proc. Natl. Acad. Sci. USA 94, 1521.
      Li, X., et al. 1997. Neuroendocrinol. 66, 63.
      Pettersson, K., et al. 1997. Mol. Endocrinol. 11, 1486.
      Pratt, W.B. and Toft, D.O. 1997. Endocrine Rev. 18, 306.
      Shughrue, P.J., et al. 1997. J. Comp. Neurol. 388, 507.
      Schumacher, M., et al. 1996. Dev. Neurosci. 18, 6.
      Toran-Allerand, C.D. 1996. Dev. Neurosci. 18, 36.
      Weigel, N.L. 1996. Biochem J. 319, 657.
      Jones, K.J. 1994. Ann. N.Y. Acad. Sci. 743, 141.
      Arnold, A.P. 1990. in Hormones, Brain and Behavior in Vertebrates, ed. Balthazart, J. (Karger, Basel), 82.
      Product Information
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
      Negative controlRecombinant human estrogen receptor α protein
      Positive controlRecombinant human estrogen receptor β
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Dot Blot
      Immunoprecipitation
      Not Western Blot
      Application NotesDot Blot (100 ng)
      Immunoprecipitation (1 µg, see comments)
      Application CommentsDoes not cross-react with estrogen receptor α. Higher molecular weight bands likely correspond to aggregation products. By dot blot, this antibody has been shown to detect recombinant human protein. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrecombinant, human estrogen receptor β
      ImmunogenHuman
      Clone10.76
      HostMouse
      IsotypeIgG
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      GR40 0

      Documentation

      Anti-Estrogen Receptor β (Ab-3) Mouse mAb (10.76) FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Anti-Estrogen Receptor β (Ab-3) Mouse mAb (10.76) Certificats d'analyse

      TitreNuméro de lot
      GR40

      Références bibliographiques

      Aperçu de la référence bibliographique
      Shughrue, P.J., et al. 1998. Endocrinol. 139, 5267.
      Al-Shamma, H.A. and Arnold, A.P. 1997. Proc. Natl. Acad. Sci. USA 94, 1521.
      Li, X., et al. 1997. Neuroendocrinol. 66, 63.
      Pettersson, K., et al. 1997. Mol. Endocrinol. 11, 1486.
      Pratt, W.B. and Toft, D.O. 1997. Endocrine Rev. 18, 306.
      Shughrue, P.J., et al. 1997. J. Comp. Neurol. 388, 507.
      Schumacher, M., et al. 1996. Dev. Neurosci. 18, 6.
      Toran-Allerand, C.D. 1996. Dev. Neurosci. 18, 36.
      Weigel, N.L. 1996. Biochem J. 319, 657.
      Jones, K.J. 1994. Ann. N.Y. Acad. Sci. 743, 141.
      Arnold, A.P. 1990. in Hormones, Brain and Behavior in Vertebrates, ed. Balthazart, J. (Karger, Basel), 82.
      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision27-August-2007 RFH
      SynonymsAnti-ER β
      ApplicationDot Blot (100 ng)
      Immunoprecipitation (1 µg, see comments)
      Application Data
      Detection of human estrogen receptor β by immunoprecipitation followed by immunoblotting. Samples: Recombinant estrogen receptor β (5 μg). Immunoprecipitating antibody: Anti-Estrogen Receptor β (Ab-3) Mouse mAb (10.76) (Cat. No. GR40) (1 μg). Conditions for immunoblotting: Samples: Recombinant estrogen receptor β (200 ng, unprecipitated) (lane A) and recombinant estrogen receptor β (immunoprecipitated above, lane B). Primary antibody: Anti-Estrogen Receptor β (Ab-2) Mouse mAb (9.88) (Cat. No. GR39) (1:1000). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Recognizes the ~53 kDa estrogen receptor β protein.
      BackgroundSteroids regulate important neuronal functions including the organization of neural circuits during development and the regulation of synapse organization in the adult. Steroid hormones may promote cell survival and differentiation of both neurons and glial cells, regulate synaptogenesis, prevent synapse elimination, cause axonal and dendritic growth, and play a role during regeneration of the nervous system. Steroid hormone receptors are members of a large family of nuclear ligand-activated transcription factors that includes the androgen, estrogen, glucocorticoid, and progesterone receptors. In the absence of hormone, steroid receptors are associated in complexes with heat shock proteins and immunophilins. Steroid binding results in the dissociation of the heat-shock protein, receptor dimerization, and binding to specific hormone response elements (HRE) to produce a transcriptionally active complex that leads to the activation or repression of target genes. Steroid receptors are regulated by phosphorylation, however, modulation of kinase activity can also cause receptor activation in the absence of hormone. Recent evidence suggests that steroid receptor expression can be regulated by neurotrophins, and that steroids can regulate the expression of trophic factors and their receptors. In addition to the classical estrogen receptor α, a second estrogen receptor, designated estrogen receptor β, has been identified. Although initially identified from the rat prostate, estrogen receptor β is expressed in a variety of tissues including the brain. In vitro studies demonstrate that estrogen receptor α and estrogen receptor β form both homodimers and heterodimers. Additionally, in situ hybridization and immunohistochemical studies indicate that estrogen receptor α and β are coexpressed in certain neuronal populations, but also exhibit distinct non-overlapping expression in the nervous system.
      HostMouse
      Immunogen speciesHuman
      Immunogenrecombinant, human estrogen receptor β
      Clone10.76
      IsotypeIgG
      Specieshuman
      Positive controlRecombinant human estrogen receptor β
      Negative controlRecombinant human estrogen receptor α protein
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsDoes not cross-react with estrogen receptor α. Higher molecular weight bands likely correspond to aggregation products. By dot blot, this antibody has been shown to detect recombinant human protein. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      +2°C to +8°C
      Do Not Freeze Ok to freeze
      Toxicity Standard Handling
      ReferencesShughrue, P.J., et al. 1998. Endocrinol. 139, 5267.
      Al-Shamma, H.A. and Arnold, A.P. 1997. Proc. Natl. Acad. Sci. USA 94, 1521.
      Li, X., et al. 1997. Neuroendocrinol. 66, 63.
      Pettersson, K., et al. 1997. Mol. Endocrinol. 11, 1486.
      Pratt, W.B. and Toft, D.O. 1997. Endocrine Rev. 18, 306.
      Shughrue, P.J., et al. 1997. J. Comp. Neurol. 388, 507.
      Schumacher, M., et al. 1996. Dev. Neurosci. 18, 6.
      Toran-Allerand, C.D. 1996. Dev. Neurosci. 18, 36.
      Weigel, N.L. 1996. Biochem J. 319, 657.
      Jones, K.J. 1994. Ann. N.Y. Acad. Sci. 743, 141.
      Arnold, A.P. 1990. in Hormones, Brain and Behavior in Vertebrates, ed. Balthazart, J. (Karger, Basel), 82.