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MAB1620-AF488 Anti-Cytokeratin 5,6 Antibody, clone D5/16B4, Alexa Fluor® 488

Anti-Cytokeratin 5,6 Antibody, clone D5/16B4, Alexa Fluor® 488 is an antibody against Cytokeratin 5,6 for use in Immunocytochemistry.

FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Research Category: Cell Structure Research Sub-Category: Cytoskeleton Gene Symbol: KRT5, DDD1, DM-EBS, EBSMCE, K-EBS, MP-EBS, WC-EBS UniProt Number: P13647
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MAB1620-AF488
100 µL  
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      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, R, HICCMAlexaFluor®488Monoclonal Antibody
      Description
      Catalogue NumberMAB1620-AF488
      DescriptionAnti-Cytokeratin 5,6 Antibody, clone D5/16B4, Alexa Fluor® 488
      Alternate Names
      • Keratin, type II cytoskeletal 5
      • 58 kDa cytokeratin
      • CK-5
      • Cytokeratin-5
      • K5
      • Keratin-5
      • Type-II keratin Kb5
      Background InformationKeratin, type II cytoskeletal 5 (UniProt P13647; also known as 58 kDa cytokeratin, CK-5, Cytokeratin-5, K5, Keratin-5, Type-II keratin Kb5) is encoded by the KRT5 (also known as DDD1, DM-EBS, EBSMCE, K-EBS, MP-EBS, WC-EBS) gene (Gene ID 3852) in human. Cytokeratin-5 (Keratin-5 or K5) belongs to a family of highly conserved epithelial-specific intermediate filament (IF) proteins. Each epithelium is characterized by its unique keratin expression pattern. In interfollicular epidermis, four major keratins are synthesized and co-polymerize in pairs, K5/K14 and K1/KI0, to form filaments. The expression of these two pairs is strictly regulated during normal keratinocyte differentiation. The K5/K14 filament is expressed in proliferating basal cells, while the K1/K10 filament is expressed during the transition to terminal differentiation and at the cessation of cell division. Similar to other IF members, Keratin-5 contains a central rod region composed of alpha helix segments (a.a. 168-203, 223-315, 339-477) that allow two different IF proteins, one type I (acidic) and one type II (basic) keratin, to intertwine into a coiled-coil formation via hydrophobic interaction. Kearain-5 is a type II keratin that pairs with the type I keratin-14 to form IF.
      References
      Product Information
      FormatAlexaFluor®488
      PresentationPurified mouse monoclonal IgG1 antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Cytokeratin 5,6 Antibody, clone D5/16B4, Alexa Fluor® 488 is an antibody against Cytokeratin 5,6 for use in Immunocytochemistry.
      Key Applications
      • Immunocytochemistry
      Application NotesThe unconjugated antibody (Cat. No. MAB1620) is shown to be suitable also for immunofluorescence, immunohistochemistry, and Western blotting applications.
      Biological Information
      ImmunogenPurified human cytokeratin 5 (Mischke, D., and Wild, G.A. (1987). J. Invest. Dermatol. 88(2):191-197; Wild, G.A., and Mischke, D. (1986). Exp. Cell Res. 162(1):114-126).
      CloneD5/16B4
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone D5/16B4 detected cytokeratin 5 by Western blotting using cytoskeletal preparations from human epidermis and non-cytokeratinizing epithelium (Abstract: European Symposium of the Biology of the Cytoskeleton, Helsinki, 18-21, 6, 89, 1989), as well as by staining tissue sections. Clone D5/16B4 also shows reactivity toward cytokeratin 6 and weak reactivity with cytokeratin 4 by Western blotting. No cross-reactivity to cytokeratin 1, 7, 8, 10, 13, 14, 18 or 19. Clone D5/16B4 immunostains basal cells and a part of the stratum spinosum in the normal pavement epithelium. Clone D5/16B4 is suitable for staining formalin-fixed tissue sections.
      IsotypeIgG1
      Species Reactivity
      • Mouse
      • Rat
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • KRT5
      • DDD1
      • DM-EBS
      • EBSMCE
      • K-EBS
      • MP-EBS
      • WC-EBS
      Purification MethodProtein A Purfied
      UniProt Number
      Molecular WeightCytokeratin 4 (59 kDa), Cytokeratin 5 (58 kDa) and Cytokeratin 6 (56 kDa).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in A431 cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Cytokeratin 5/6 in A431 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      MAB1620-AF488 04055977331875

      Documentation

      Anti-Cytokeratin 5,6 Antibody, clone D5/16B4, Alexa Fluor® 488 FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Anti-Cytokeratin 5,6 Antibody, clone D5/16B4, Alexa Fluor® 488 Certificats d'analyse

      TitreNuméro de lot
      Anti-Cytokeratin 5,6, clone D5/16B4, -Q2663597 Q2663597
      Anti-Cytokeratin 5,6, clone D5/16B4, Alexa Fluor® 488 - 2913378 2913378
      Anti-Cytokeratin 5,6, clone D5/16B4, Alexa Fluor® 488 - 3433920 3433920
      Anti-Cytokeratin 5,6, clone D5/16B4, Alexa Fluor® 488 - 4082551 4082551
      Anti-Cytokeratin 5,6, clone D5/16B4, Alexa Fluor® 488 Monoclonal Antibody 3133544

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      Catégories

      Life Science Research > Antibodies and Assays > Primary Antibodies