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MAB1910 Anti-Collagen Type IV α 2 Chain Antibody, clone 23IIC3

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MAB1910
100 µL  
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      Tableau de caractéristiques principal

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Mk, RELISA, IH(P), WBMAscitesMonoclonal Antibody
      Description
      Catalogue NumberMAB1910
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Collagen Type IV α 2 Chain Antibody, clone 23IIC3
      Product Information
      FormatAscites
      Control
      • Positive Control: Kidney, muscle, tendon spleen tissue
        Negative Control: Neurons/glia
      PresentationLiquid.
      Quality LevelMQ100
      Applications
      ApplicationDetect Collagen Type IV α 2 Chain using this Anti-Collagen Type IV α 2 Chain Antibody, clone 23IIC3 validated for use in ELISA, IH(P) & WB.
      Key Applications
      • ELISA
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry: 1:500, acetone fixed, frozen tissue recommended. Weak reactivity on on formalin fixed, paraffin sections (1:100) after treatment with 1% trypsin (20 minutes, room temperature). Or alternatively, 4% PFA fixed, HEIR treated tissue has worked in monkey (Qin, 2003) at 1:200 dilution.

      Western blot: 1:500, 170 kDa.

      ELISA at 1:200

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenHuman amniotic type IV collagen
      Clone23IIC3
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes Human type IV collagen, alpha-2 chains.
      IsotypeIgG1
      Species Reactivity
      • Human
      • Monkey
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene SummaryThis gene encodes the major type IV alpha collagen chain of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter.
      Gene Symbol
      • COL4A2
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight170 kDa
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µL
      Global Trade Item Number
      Référence GTIN
      MAB1910 04053252617416