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AB144P Anti-Choline Acetyltransferase Antibody

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AB144P
500 µL  
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      Tableau de caractéristiques principal

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, M, Mk, Op, Av, Ch, Gp, ZebrafishIH(P), ICC, IHC, WBGtAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB144P
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Choline Acetyltransferase Antibody
      OverviewCatalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
      Alternate Names
      • Choline acetylase
      • acetyl CoA:choline O-acetyltransferase
      • choline acetyltransferase
      • ChAT
      Background InformationAcetylcholine (ACh) is a common neurotransmitter for motoneurons, preganglionic autonomic neurons, postganglionic parasympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells. The metabolism of Ach is relatively simple, involving only two enzymes: choline acetyltransferase (ChAT) for synthesis and acetylcholinesterase (AChE) for degradation. Further, acetylcholine has little function in neurons other than neurotransmission and seems to be neuron specific. It seems that only cholinergic neurons have significant amounts of ChAT making anti-choline acetyltransferase a useful specific marker.
      Product Information
      FormatAffinity Purified
      Control
      • Human placenta lysates, rat forebrain/rostral hypothalamus.
      PresentationPurified goat polyclonal in buffer containing 5 mg/mL BSA and 0.2% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Choline Acetyltransferase Antibody detects level of ChAT and has been published and validated for use in IH(P), IC, IH and WB.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Immunocytochemistry
      • Immunohistochemistry
      • Western Blotting
      Application NotesImmunohistochemistry:
      A previous lot of this antibody was used at 1:100.

      Optimal working dilutions and protocols must be determined by end user.
      Biological Information
      ImmunogenHuman placental enzyme.
      HostGoat
      SpecificityCholine acetyltransferase found in cholinergic neurons in brain and spinal cord.
      Species Reactivity
      • Human
      • Rat
      • Mouse
      • Monkey
      • Opossum
      • Avian
      • Chicken
      • Guinea Pig
      • Zebrafish
      Species Reactivity NoteHuman and mouse. Expected to cross-react with Avian, chicken, guinea pig, monkey, rat, and opposum.
      Demonstrated to react in zebrafish in THE JOURNAL OF COMPARATIVE NEUROLOGY 474:75–107 (2004).
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryCholinergic systems are implicated in numerous neurologic functions. Alteration in some cholinergic neurons may account for the disturbances of Alzheimer disease. The protein encoded by this gene synthesizes the neurotransmitter acetylcholine. Alternative splice variants have been found that contain alternative 5' untranslated exons. Three of the four described splice variants encode identical 69 kDa proteins while one variant encodes both the 69 kDa and a larger 82 kDa protein.
      Gene Symbol
      • CHAT
      • ChAT
      • CHOACTase
      • CMS1A
      • CMS1A2
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P28329 # Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
      SIZE: 748 amino acids; 82568 Da
      DISEASE: SwissProt: P28329 # Defects in CHAT are the cause of familial infantile myasthenia gravis 2 (FIMG2) [MIM:254210, 254200]; also known as CMS-EA. FIMG2 patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement. Inheritance is autosomal recessive.
      SIMILARITY: SwissProt: P28329 ## Belongs to the carnitine/choline acetyltransferase family.
      Molecular Weight70/74 kDa
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on mouse brain lysates.

      Western Blot Analysis:
      1:1000 dilution of this lot detected CHAT on 10 μg of mouse brain lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 1 year from date of receipt. Avoid repeated freeze/thaw cycles.
      For short term storage (3-4 weeks) maintain at 2-8°C.
      Packaging Information
      Material Size500 µL
      Global Trade Item Number
      Référence GTIN
      AB144P 08436037123283