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AB81023 Anti-Calpain II Antibody, large subunit

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AB81023
100 µl  
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      Tableau de caractéristiques principal

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, M, HWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB81023
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Calpain II Antibody, large subunit
      Alternate Names
      • M-Calpain
      • Calpain 2
      Background InformationCalpains are cytosolic, calcium-dependent, cysteine proteases that are ubiquitously expressed in mammalian and avian tissues. Calpain-1 and 2 consist of a common small subunit and a large variable subunit. Domains in the large subunit include the amino terminal domain I, the proteinase domain II, domain III, and the EF-hand domain IV.
      Type I calpain requires micromolar amounts of calcium, while type II calpain requires millimolar amounts of calcium.
      References
      Product Information
      FormatAffinity Purified
      PresentationAffinity purified immunoglobulin. Liquid in PBS pH 7.4 containing 1% BSA and 50% glycerol as a stabilizer.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Calpain II Antibody, large subunit is an antibody against Calpain II for use in WB.
      Key Applications
      • Western Blotting
      Application NotesWestern blot: 1:1000 for colorimetric substrates or 1:5000 for chemiluminescent substrates (recommended starting concentration).

      Optimal working dilutions must be determined by the end user.
      Biological Information
      Epitopelarge subunit
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThe anti-calpain II antibody recognizes the 80 kDa and 58 kDa forms of Calpain II. It does not cross-react with other calpains.
      Species Reactivity
      • Rat
      • Mouse
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported.
      Gene Symbol
      • CAPN2
      • millimolar-calpain
      • M-calpain
      • Millimolar-calpain
      • mCANP
      • FLJ39928
      • CANP2
      • CANPml
      • CANPL2
      • EC 3.4.22.53
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P17655 # Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
      COFACTOR: Binds 3 calcium ions.
      SIZE: 700 amino acids; 80009 Da
      SUBUNIT: Forms a heterodimer with a small (regulatory) subunit (CAPNS1).
      SUBCELLULAR LOCATION: Cytoplasm. Cell membrane. Note=Translocates to the plasma membrane upon Ca(2+) binding.
      TISSUE SPECIFICITY: Ubiquitous.
      SIMILARITY: SwissProt: P17655 ## Belongs to the peptidase C2 family. & Contains 1 calpain catalytic domain. & Contains 3 EF-hand domains.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20*C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µl
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      AB81023 04053252286544

      Documentation

      Required Licenses

      Title
      PRODUCTO REGULADO POR LA SECRETARÍA DE SALUD

      Anti-Calpain II Antibody, large subunit FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Anti-Calpain II Antibody, large subunit Certificats d'analyse

      TitreNuméro de lot
      RABBIT ANTI-CALPAIN II (m-Calpain) LARGE SUBUNIT 2462753
      RABBIT ANTI-CALPAIN II (m-Calpain) LARGE SUBUNIT - AB81023 AB81023
      RABBIT ANTI-CALPAIN II (m-Calpain) LARGE SUBUNIT -2705110 2705110
      RABBIT ANTI-CALPAIN II (m-Calpain) LARGE SUBUNIT -2707858 2707858
      RABBIT ANTI-CALPAIN II (m-Calpain) LARGE SUBUNIT POLYCLONAL ANTIBODY 3076497
      RABBIT ANTI-CALPAIN II (m-Calpain) LARGE SUBUNIT POLYCLONAL ANTIBODY 3035352
      RABBIT ANTI-CALPAIN II (m-Calpain) LARGE SUBUNIT POLYCLONAL ANTIBODY -2571247 2571247
      RABBIT ANTI-CALPAIN II (m-Calpain) LARGE SUBUNIT POLYCLONAL ANTIBODY -2781090 2781090

      Références bibliographiques

      Aperçu de la référence bibliographiqueApplicationEspèceNº PubMed
      Calpain cleavage and inactivation of the sodium calcium exchanger-3 occur downstream of Aβ in Alzheimer's disease.
      Atherton, J; Kurbatskaya, K; Bondulich, M; Croft, CL; Garwood, CJ; Chhabra, R; Wray, S; Jeromin, A; Hanger, DP; Noble, W
      Aging cell  13  49-59  2014

      Afficher le résumé
      Western Blotting23919677 23919677
      N-methyl-D-aspartate receptor- and calpain-mediated proteolytic cleavage of K+-Cl- cotransporter-2 impairs spinal chloride homeostasis in neuropathic pain.
      Zhou, HY; Chen, SR; Byun, HS; Chen, H; Li, L; Han, HD; Lopez-Berestein, G; Sood, AK; Pan, HL
      The Journal of biological chemistry  287  33853-64  2011

      Afficher le résumé
      Western BlottingRat22854961 22854961
      Calpain and PARP activation during photoreceptor cell death in P23H and S334ter rhodopsin mutant rats.
      Kaur, J; Mencl, S; Sahaboglu, A; Farinelli, P; van Veen, T; Zrenner, E; Ekström, P; Paquet-Durand, F; Arango-Gonzalez, B
      PloS one  6  e22181  2010

      Afficher le résumé
      21765948 21765948
      Brain-derived neurotrophic factor and epidermal growth factor activate neuronal m-calpain via mitogen-activated protein kinase-dependent phosphorylation.
      Zadran, S; Jourdi, H; Rostamiani, K; Qin, Q; Bi, X; Baudry, M
      The Journal of neuroscience : the official journal of the Society for Neuroscience  30  1086-95  2009

      Afficher le résumé Article en texte intégral
      20089917 20089917