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MABF2761-25UG Anti-CXCL1/GROa Antibody, clone HL2401

Anti-CXCL1/GROa, clone HL2401, Cat. No. MABF2761, is a mouse monoclonal antibody that detects CXCL1 and is tested for use in ELISA, Immunoprecipitation, Inhibition Assay, and Western Blotting.

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MABF2761-25UG
25 μg  
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      Replacement Information
      Description
      Catalogue NumberMABF2761-25UG
      DescriptionAnti-CXCL1/GROa Antibody, clone HL2401
      Alternate Names
      • C-X-C motif chemokine 1
      • GRO-alpha(1-73)
      • Melanoma growth stimulatory activity
      • MGSA
      • Neutrophil-activating protein 3
      • NAP-3
      Background InformationGrowth-regulated alpha protein (UniProt: P09341; also known as C-X-C motif chemokine 1, CXCL1, GRO-alpha(1-73), Melanoma growth stimulatory activity, MGSA, Neutrophil-activating protein 3, NAP-3) is encoded by the CXCL1 (also known as GRO, GRO1, GROA, MGSA, SCYB1) gene (Gene ID: 2919) in human. CXCL1 is a multi-functional chemokine that displays chemotactic activity for neutrophils. It is synthesized with a signal peptide (aa 1-34), which is subsequently cleaved off to produce the mature protein that can be further cleaved into three chains (aa 4-73; 5-73; and 6-73) via proteolytic cleavage after secretion from peripheral blood monocytes. CXCL1 may play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. Its levels are reported to be low under basal conditions but increase significantly following active infection. It plays a role in recruiting and activating neutrophils for microbial killing at the tissue site and can also activate the release of proteases and reactive oxygen species. CXCL1 signals through binding to CXCR2, a G-protein coupled receptor. Aberrant expression of CXCL1 has been reported in several cancers and has been associated with oncogenesis, metastasis, angiogenesis and chemoresistance. Clone HL2401 is shown to specifically binds to CXCL1, although it may show slight binding to CXCL2. It is shown to reduce both cellular proliferation and invasion of cancer cells overexpressing CXCL1. It also induces apoptosis and impairs tumor vasculature. (Ref.: Miyake, M., et al. (2019). Theranostics.;9(3):853-867; Yang C., et al. (2019). Int. J. Oncol. 55(3); 684-696Wuyts, A., et al. (1999). Eur. J. Biochem. 260(2); 421-429).
      References
      Product Information
      FormatPurified
      Control
      • MilliporeSigma offers a comprehensive range of trusted and highly cited primary and secondary antibodies for your research needs. Explore our 94
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      • application
      • species reactivity
      • host species and more.
      PresentationPurified mouse monoclonal antibody IgG1 in PBS with 0.02% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-CXCL1/GROa, clone HL2401, Cat. No. MABF2761, is a mouse monoclonal antibody that detects CXCL1 and is tested for use in ELISA, Immunoprecipitation, Inhibition Assay, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Inhibition
      • ELISA
      Application NotesTested applications

      ELISA Analysis: A representative lot detected CXCL1 in ELISA application (Miyake, M., et al. (2019). Theranostics. 9(3):853-867).

      Inhibition Assay: A representative lot inhibited proliferation and invasion of bladder and prostate cells and disrupted endothelial sprouting in vitro. (Miyake, M., et al. (2019). Theranostics. 9(3):853-867).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated CXCL1 in Immunoprecipitation application (Miyake, M., et al. (2019). Theranostics. 9(3):853-867).

      Western Blotting Analysis: A representative lot detected CXCL1 in Western Blotting application (Miyake, M., et al. (2019). Theranostics. 9(3):853-867).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenNative human CXCL1 protein.
      EpitopeUnknown
      CloneHL2401
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone HL2401 is a mouse monoclonal antibody that detects CXCL1.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CXCL1
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~15 kDa observed; 11.3 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting with recombinant Human CXCL1 protein.

      Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected recombinant Human CXCL1 protein.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      MABF2761-25UG 04065269049241