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AP1169 Anti-CCL7 Mouse mAb (4B5)

Anti-CCL7 Mouse mAb (4B5) : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.

Synonymes: Anti-Monocyte Chemotactic Protein 3, Anti-Small Inducible Cytokine A7, Anti-Chemokine Ligand 7

AP1169
  
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      Recognizes the CCL7 protein.
      Catalogue NumberAP1169
      Brand Family Calbiochem®
      SynonymsAnti-Monocyte Chemotactic Protein 3, Anti-Small Inducible Cytokine A7, Anti-Chemokine Ligand 7
      Application Data
      Detection of human CCL7 by immunoblotting. Sample: Whole cell lysate from 293T cells, transfected with partial recombinant, human CCL7 (lane 1) and from non-transfected 293T cells (lane 2). Primary antibody: Anti-CCL7 Mouse mAb (4B5) (Cat. No. AP1169) (1-5 µg/ml). Detection: chemiluminescence.
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids of 24-100 human CCL7, expressed as a GST fusion protein
      Clone4B5
      HostMouse
      IsotypeIgG2b
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      AP1169 0

      Documentation

      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision19-October-2010 RFH
      SynonymsAnti-Monocyte Chemotactic Protein 3, Anti-Small Inducible Cytokine A7, Anti-Chemokine Ligand 7
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application Data
      Detection of human CCL7 by immunoblotting. Sample: Whole cell lysate from 293T cells, transfected with partial recombinant, human CCL7 (lane 1) and from non-transfected 293T cells (lane 2). Primary antibody: Anti-CCL7 Mouse mAb (4B5) (Cat. No. AP1169) (1-5 µg/ml). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the CCL7 protein.
      BackgroundCCL7 is a monocyte chemotactic protein 3, a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix.
      HostMouse
      ImmunogenA recombinant polypeptide corresponding to amino acids of 24-100 human CCL7, expressed as a GST fusion protein
      Clone4B5
      IsotypeIgG2b
      Specieshuman
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review