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MAB3222 Anti-BrdU Antibody, clone IIB5

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MAB3222
100 µg  
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      Tableau de caractéristiques principal

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      AFC, ICC, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB3222
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-BrdU Antibody, clone IIB5
      Alternate Names
      • BrdU
      Background InformationThe immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
      Product Information
      FormatPurified
      Control
      • After incorporation of BrdU, all DNA containing species
      PresentationProtein A Purified mouse immunoglobulin in PBS with 0.09% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-BrdU Antibody, clone IIB5 is a Mouse Monoclonal Antibody for detection of Bromodeoxyuridine also known as BrdU & has been validated in FC, ICC, IHC(P).
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry: 1:100-1:200 on frozen and paraffin embedded tissues with acid/proteinase treatments. See:

      http://www.protocol-online.org/prot/Immunology/Immunohistochemistry. For examples of various protocols.

      Flow Cytometry: 1:100-1:200; acid methanol treatment required.

      Optimal working dilutions must be determined by the end user.

      In order for the antibody to work a pretreatment to expose DNA will be needed (1 mg/mL of pepsin in 0.01 M HCL 30 min at r/t)
      Biological Information
      ImmunogenA fusion of mouse myeloma cells with spleen cells from a mouse intraperitoneally immunized with BrdU conjugated to bovine serum albumin.
      CloneIIB5
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityReacts specifically with bromodeoxyuridine. It also recognizes BrdU when incorporated into nuclear DNA.
      IsotypeIgG1
      Species Reactivity
      • All
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein A Purfied
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Référence GTIN
      MAB3222 04053252666414