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NA20 Anti-BrdU (Ab-2) Mouse mAb (ZBU30)

Anti-BrdU (Ab-2) Mouse mAb (ZBU30) : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.

Synonymes: Anti-Bromodeoxyuridine

NA20
  
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      Aperçu

      Replacement Information

      Tableau de caractéristiques principal

      Host
      M
      Description
      OverviewRecognizes BrdU (bromodeoxyuridine). Does not cross-react with thymidine. Use of this antibody requires biosynthetic labeling of the target tissues or cells with the bromodeoxyuridine analog such as 5-Bromo-2′-deoxyuridine (Cat. No. 203806).
      Catalogue NumberNA20
      Brand Family Calbiochem®
      SynonymsAnti-Bromodeoxyuridine
      References
      ReferencesSchutte, B. et al. 1987. J. Histochem. Cytochem. 35, 1343. Gratzner, H.G. 1982. Science 218, 474. Gratzner, H.G. and Leif, R.C. 1981. Cytometry 1, 385. Gratzner, H.G. et al. 1975. Exp. Cell Res. 95, 88.
      Product Information
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
      Positive controlBrdU-labeled cells
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Frozen Sections
      Immunofluorescence
      Paraffin Sections
      Application NotesFlow cytometry (see comments) Frozen Sections (2 µg/ml) Immunofluorescence (see comments) Paraffin Sections (2.5 µg/ml, trypsin pre-treatment required)
      Application CommentsUse of this antibody first requires biosynthetic labeling of the target tissues or cells with bromodeoxyuridine (Cat. No. 203806). The DNA must be denatured in order for the BrdU to be detected. This antibody will cross-react with IdU. Cultured cells can be pulse labeled with 10 µM bromodeoxyuridine for 30 min. For whole tissues, animals can be injected with 50 mg BrdU/kg body weight, sacrificed 1 h later, and the target organ(s) removed. This antibody is also reported to work for flow cytometry and immunofluorescence. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      ImmunogenBrdU-labeled DNA
      CloneZBU30
      HostMouse
      IsotypeIgG₁
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      NA20 0

      Documentation

      Anti-BrdU (Ab-2) Mouse mAb (ZBU30) FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Anti-BrdU (Ab-2) Mouse mAb (ZBU30) Certificats d'analyse

      TitreNuméro de lot
      NA20

      Références bibliographiques

      Aperçu de la référence bibliographique
      Schutte, B. et al. 1987. J. Histochem. Cytochem. 35, 1343. Gratzner, H.G. 1982. Science 218, 474. Gratzner, H.G. and Leif, R.C. 1981. Cytometry 1, 385. Gratzner, H.G. et al. 1975. Exp. Cell Res. 95, 88.

      Brochure

      Titre
      Caspases and other Apoptosis Related Tools Brochure
      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision05-September-2007 RFH
      SynonymsAnti-Bromodeoxyuridine
      ApplicationFlow cytometry (see comments) Frozen Sections (2 µg/ml) Immunofluorescence (see comments) Paraffin Sections (2.5 µg/ml, trypsin pre-treatment required)
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 myeloma cells. Recognizes BrdU-labeled DNA.
      BackgroundProliferating cells are usually detected by incorporation of [3H]thymidine into DNA and quantitation by autoradiography or scintillation counting. Alternatively, bromodeoxyuridine (BrdU), an analog of thymidine, can be incorporated specifically into the DNA of replicating cells and the extent of incorporation can be detected using antibodies directed against the incorporated analog. The proportion of cells actively proliferating can then be determined by immunofluorescence or flow cytometry as well as immunohistochemistry (which has been done with tumors, tissues, and blood cell elements).
      HostMouse
      ImmunogenBrdU-labeled DNA
      CloneZBU30
      IsotypeIgG₁
      Speciesa broad range of species
      Positive controlBrdU-labeled cells
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsUse of this antibody first requires biosynthetic labeling of the target tissues or cells with bromodeoxyuridine (Cat. No. 203806). The DNA must be denatured in order for the BrdU to be detected. This antibody will cross-react with IdU. Cultured cells can be pulse labeled with 10 µM bromodeoxyuridine for 30 min. For whole tissues, animals can be injected with 50 mg BrdU/kg body weight, sacrificed 1 h later, and the target organ(s) removed. This antibody is also reported to work for flow cytometry and immunofluorescence. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesSchutte, B. et al. 1987. J. Histochem. Cytochem. 35, 1343. Gratzner, H.G. 1982. Science 218, 474. Gratzner, H.G. and Leif, R.C. 1981. Cytometry 1, 385. Gratzner, H.G. et al. 1975. Exp. Cell Res. 95, 88.

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      Catégories

      Life Science Research > Antibodies and Assays > Primary Antibodies