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OP92 Anti-BRCA1 (Ab-1) Mouse mAb (MS110)

Anti-BRCA1 (Ab-1), mouse monoclonal, clone MS110, recognizes the ~220 kDa BRCA1 protein in MCF-7 cells. It is validated for WB, IF, IP, and frozen and paraffin sections.

Anti-BRCA1 (Ab-1) Mouse mAb (MS110) : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.

Synonymes: Anti-Breast Cancer Susceptibility Protein

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OP92
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Tableau de caractéristiques principal

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

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OP92-100UG
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      Description
      OverviewRecognizes the ~220 kDa BRCA1 protein in MCF-7 cells. Does not cross-react with blood group antigens or growth factor receptors such as EGFR. Sold under license of U.S. Patent 5,753,441 and 6,162,897.
      Catalogue NumberOP92
      Brand Family Calbiochem®
      SynonymsAnti-Breast Cancer Susceptibility Protein
      References
      ReferencesScully, R., et al. 1996. Science 272, 123.
      Gudas, J.M., et al. 1996. Cell Growth Differ. 7, 717.
      Vaughn, J.P., et al. 1996. Cell Growth Differ. 7, 711.
      Goldgar, D.E. and Reilly, P.R. 1995. Nat. Genet. 11, 113.
      Merajver, S.D., et al. 1995. Clin. Can. Res. 1, 539.
      Merajver, S.D., et al. 1995. Nat. Genet. 9, 439.
      Struewing, J.P., et al. 1995. Nat. Genet. 11, 198.
      Thompson, M.E., et al. 1995. Nat. Genet. 9, 444.
      Futreal, P.A., et al. 1994. Science 266, 120.
      Miki, Y., et al. 1994. Science 266, 66.
      Wilson, C.A., et al. 1999. Nat. Genet. 21, 236.
      Product Information
      DeclarationSold under license of U.S. Patents 5,753,441 and 6,162,897.
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
      Positive controlMCF7 cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesImmunoblotting Wilson, C.A., et al. 1999. Nature Genetics 21, 236. Scully, R., et al. 1996. Science 272, 123. Immunoprecipitation, Immunofluorescence Wilson, C.A., et al. 1999. Nature Genetics 21, 236. Scully, R., et al. 1996. Science 272, 123. Frozen Sections, Paraffin Sections Wilson, C.A., et al. 1999. Nature Genetics 21, 236.
      Key Applications Frozen Sections
      Immunoblotting (Western Blotting)
      Immunofluorescence
      Immunoprecipitation
      Paraffin Sections
      Application NotesFrozen Sections (see application reference)
      Immunoblotting (2 µg/ml)
      Immunofluorescence (see application reference)
      Immunoprecipitation (see application reference)
      Paraffin Sections (see application reference)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrecombinant human BRCA1
      ImmunogenHuman
      Epitopewithin the N-terminal 304 amino acids of BRCA1
      CloneMS110
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      OP92-100UG 04055977209686

      Documentation

      Anti-BRCA1 (Ab-1) Mouse mAb (MS110) Certificats d'analyse

      TitreNuméro de lot
      OP92

      Références bibliographiques

      Aperçu de la référence bibliographique
      Scully, R., et al. 1996. Science 272, 123.
      Gudas, J.M., et al. 1996. Cell Growth Differ. 7, 717.
      Vaughn, J.P., et al. 1996. Cell Growth Differ. 7, 711.
      Goldgar, D.E. and Reilly, P.R. 1995. Nat. Genet. 11, 113.
      Merajver, S.D., et al. 1995. Clin. Can. Res. 1, 539.
      Merajver, S.D., et al. 1995. Nat. Genet. 9, 439.
      Struewing, J.P., et al. 1995. Nat. Genet. 11, 198.
      Thompson, M.E., et al. 1995. Nat. Genet. 9, 444.
      Futreal, P.A., et al. 1994. Science 266, 120.
      Miki, Y., et al. 1994. Science 266, 66.
      Wilson, C.A., et al. 1999. Nat. Genet. 21, 236.

      Citations

      Titre
    • Richard D. Kennedy, et al. (2005) BRCA1 and c-Myc associate to transcriptionally repress psoriasin, a DNA damage-inducible gene. Cancer Research 65, 10265-10272.
      		•
      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision03-October-2007 RFH
      SynonymsAnti-Breast Cancer Susceptibility Protein
      ApplicationFrozen Sections (see application reference)
      Immunoblotting (2 µg/ml)
      Immunofluorescence (see application reference)
      Immunoprecipitation (see application reference)
      Paraffin Sections (see application reference)
      DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells. Recognizes the ~220 kDa BRCA1 protein.
      BackgroundInactivation of the BRCA1 gene, located at 17q21, is responsible for some forms of familial breast and ovarian cancer and sporadic ovarian cancer. BRCA1 encodes a protein of 1863 amino acids with an amino-terminal domain that exhibits similarity to the zinc-ring domain found in several regulatory and transcription factors. Mutations that disrupt this domain are some of the most frequently occurring ones linked to breast and ovarian cancer. Inhibition of BRCA1 expression leads to accelerated growth of mammary cells in vitro while loss of expression is observed during progression from benign disease to metastatic breast cancer suggesting that BRCA1 serves as a negative growth regulator. The ~220 kDa BRCA1 protein is expressed predominantly in the nucleus and appears to undergo cell cycle regulation with mRNA expression induced prior to DNA synthesis.
      HostMouse
      Immunogen speciesHuman
      Immunogenrecombinant human BRCA1
      Epitopewithin the N-terminal 304 amino acids of BRCA1
      CloneMS110
      IsotypeIgG₁
      Specieshuman
      Positive controlMCF7 cells
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesScully, R., et al. 1996. Science 272, 123.
      Gudas, J.M., et al. 1996. Cell Growth Differ. 7, 717.
      Vaughn, J.P., et al. 1996. Cell Growth Differ. 7, 711.
      Goldgar, D.E. and Reilly, P.R. 1995. Nat. Genet. 11, 113.
      Merajver, S.D., et al. 1995. Clin. Can. Res. 1, 539.
      Merajver, S.D., et al. 1995. Nat. Genet. 9, 439.
      Struewing, J.P., et al. 1995. Nat. Genet. 11, 198.
      Thompson, M.E., et al. 1995. Nat. Genet. 9, 444.
      Futreal, P.A., et al. 1994. Science 266, 120.
      Miki, Y., et al. 1994. Science 266, 66.
      Wilson, C.A., et al. 1999. Nat. Genet. 21, 236.
      Citation
    • Richard D. Kennedy, et al. (2005) BRCA1 and c-Myc associate to transcriptionally repress psoriasin, a DNA damage-inducible gene. Cancer Research 65, 10265-10272.
      		•
      Application referencesImmunoblotting Wilson, C.A., et al. 1999. Nature Genetics 21, 236. Scully, R., et al. 1996. Science 272, 123. Immunoprecipitation, Immunofluorescence Wilson, C.A., et al. 1999. Nature Genetics 21, 236. Scully, R., et al. 1996. Science 272, 123. Frozen Sections, Paraffin Sections Wilson, C.A., et al. 1999. Nature Genetics 21, 236.