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ST1531 Anti-ADH4 Mouse mAb (1D2)

Anti-ADH4 Mouse mAb (1D2) : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
ST1531
  
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      Description
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      This product has been discontinued.





      Recognizes the ~37 kDa ADH4 protein in human liver tissue and HeLa NE cells.
      Catalogue NumberST1531
      Brand Family Calbiochem®
      Application Data
      Detection of human ADH4 by immunoblotting. Sample: Whole tissue extract from human liver. Primary antibody: Anti-ADH4 Mouse mAb (1D2) (Cat. No. ST1531) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human ADH4 by immunoblotting. Sample: Whole cell lysate from HeLa NE cells. Primary antibody: Anti-ADH4 Mouse mAb (1D2) (Cat. No. ST1531) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human ADH4 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human liver tissue. Primary antibody: Anti-ADH4 Mouse mAb (1D2) (Cat. No. ST1531) (3 µg/ml). Detection: chemiluminescence.
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Positive controlHuman liver tissue, HeLa NE cells
      PreservativeNone
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Paraffin Sections (3 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids 52-151 of human ADH4, expressed as a GST fusion protein
      Clone1D2
      HostMouse
      IsotypeIgG2a
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      ST1531 0

      Documentation

      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision08-November-2010 RFH
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Paraffin Sections (3 µg/ml)
      Application Data
      Detection of human ADH4 by immunoblotting. Sample: Whole tissue extract from human liver. Primary antibody: Anti-ADH4 Mouse mAb (1D2) (Cat. No. ST1531) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human ADH4 by immunoblotting. Sample: Whole cell lysate from HeLa NE cells. Primary antibody: Anti-ADH4 Mouse mAb (1D2) (Cat. No. ST1531) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human ADH4 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human liver tissue. Primary antibody: Anti-ADH4 Mouse mAb (1D2) (Cat. No. ST1531) (3 µg/ml). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~37 kDa ADH4 protein.
      BackgroundADH4 is a class II alcohol dehydrogenase 4 pi subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class II alcohol dehydrogenase is a homodimer composed of 2 pi subunits. It exhibits a high activity for oxidation of long-chain aliphatic alcohols and aromatic alcohols and is less sensitive to pyrazole.
      HostMouse
      ImmunogenA recombinant polypeptide corresponding to amino acids 52-151 of human ADH4, expressed as a GST fusion protein
      Clone1D2
      IsotypeIgG2a
      Specieshuman
      Positive controlHuman liver tissue, HeLa NE cells
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review