Millipore Sigma Vibrant Logo

NA02A Anti-2,2,7-Trimethylguanosine Mouse mAb (K121) Agarose Conjugate

This Anti-2,2,7-Trimethylguanosine Mouse mAb (K121) Agarose Conjugate is validated for use in Affinity Purification, IP, IP, ICC, Paraffin Sections for the detection of 2,2,7-Trimethylguanosine.

FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
View Products on Sigmaaldrich.com
NA02A
Voir les Prix & la Disponibilité

Aperçu

Replacement Information

Tableau de caractéristiques principal

Species ReactivityHostAntibody Type
H, M, RMMonoclonal Antibody

Prix & Disponibilité

Référence DisponibilitéConditionnement Qté Prix Quantité
NA02A-0.5ML
Récupération des données relatives à la disponibilité...
—
Disponibilité limitée Disponibilité limitée
En stock 
Interrompu(e)
Disponible en quantités limitées
Disponibilité à confirmer
    Pour le restant : Nous vous tiendrons informé
      Pour le restant : Nous vous tiendrons informé
      Nous vous tiendrons informé
      Contacter le Service Clients
      Contact Customer Service

      		 Ampoule plast. 0.5 ml
      Prix en cours de récupération
      Le prix n'a pas pu être récupéré
      La quantité minimale doit être un multiple de
      Maximum Quantity is
      À la validation de la commande Plus d'informations
      Vous avez sauvegardé ()
       
      —
      Demander le prix
      		 —
      Description
      OverviewRecognizes the exposed 5′ cap structure (2,2,7-trimethylguanosine) of small nuclear RNAs (snRNAs). Also recognizes 7-methylguanosine-capped mRNA.
      Catalogue NumberNA02A
      Brand Family Calbiochem®
      References
      ReferencesCasciola-Rosen, L.A., et al. 1994. J. Biol. Chem. 269, 30757-30760.
      Krainer, A.R. 1988. Nuc. Acids Res. 16, 9415.
      Luhrmann, R. 1988. Birntiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 71.
      Reddy, R. and Busch, H., 1988. Birnstiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 1.
      Krainer, A.R. and Maniatis, T. 1985. Cell 42, 725.
      Kramer, A., et al. 1984. Cell 38, 299.
      Lerner, M.R., et al. 1980. Nature 283, 220.
      Product Information
      FormLiquid suspension
      FormulationIn PBS.
      Negative controlTransfer RNA (tRNA)
      Positive controlHeterogenous nuclear RNA (hnRNA)
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesAffinity Purification Luhrmann, R. 1988. Birntiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 71. Immunoprecipitation Moteki, S. and Price, D. 2002. Mol. Cell 10, 599.
      Key Applications Affinity Purification
      Immunoblotting (Western Blotting)
      Application NotesAffinity Purification (see application references)
      Immunoprecipitation (15 µl beads/sample)
      Immunoprecipitation (1-2 µg/ml; see application references)
      Immunocytochemistry (use Cat. No. NA02)
      Paraffin Sections (use Cat. No. NA02)
      Application CommentsAlso recognizes 7-methylguanosine-capped mRNA. 2,2,7-trimethylguanosine (Ab-1) is human, mouse, and rat reactive. This antibody reacts with the exposed 5' cap structure of the snRNAs and the antibody can be used for the purification of snRNPs by immunoaffinity chromatography. All of the major snRNPs bind to the monoclonal antibody column and can be gently eluted by washing with 7-methyl guanosine. Antibody should be titrated for optimal results in individual sample types.
      Biological Information
      Immunogen2,2,7-trimethylguanosine
      CloneK121
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      NA02A-0.5ML 04055977227611

      Documentation

      Anti-2,2,7-Trimethylguanosine Mouse mAb (K121) Agarose Conjugate FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Anti-2,2,7-Trimethylguanosine Mouse mAb (K121) Agarose Conjugate Certificats d'analyse

      TitreNuméro de lot
      NA02A

      Références bibliographiques

      Aperçu de la référence bibliographique
      Casciola-Rosen, L.A., et al. 1994. J. Biol. Chem. 269, 30757-30760.
      Krainer, A.R. 1988. Nuc. Acids Res. 16, 9415.
      Luhrmann, R. 1988. Birntiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 71.
      Reddy, R. and Busch, H., 1988. Birnstiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 1.
      Krainer, A.R. and Maniatis, T. 1985. Cell 42, 725.
      Kramer, A., et al. 1984. Cell 38, 299.
      Lerner, M.R., et al. 1980. Nature 283, 220.

      Brochure

      Titre
      Antibody Sourcebookﾙ Sixth Edition
      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision04-September-2007 RFH
      ApplicationAffinity Purification (see application references)
      Immunoprecipitation (15 µl beads/sample)
      Immunoprecipitation (1-2 µg/ml; see application references)
      Immunocytochemistry (use Cat. No. NA02)
      Paraffin Sections (use Cat. No. NA02)
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Conjugated to agarose. Recognizes the exposed 5' cap structure (2,2,7-trimethylguanosine) of small nuclear RNAs (snRNAs).
      BackgroundThe U small nuclear ribonucleoprotein particles (snRNPs) are present in all eukaryotes studied thus far and are involved in the splicing of RNA molecules. snRNPs are composed of several proteins and one or two RNA molecules. The RNA molecules (snRNAs) contain a unique 5' terminal cap structure containing the nucleoside 2,2,7-trimethylguanosine. The 70 kDa protein component of U1 snRNP is cleaved during apoptosis, perhaps by a caspase. Monoclonal antibodies to the 5' cap structure react only to snRNAs.
      HostMouse
      Immunogen2,2,7-trimethylguanosine
      CloneK121
      IsotypeIgG₁
      Specieshuman, mouse, rat
      Positive controlHeterogenous nuclear RNA (hnRNA)
      Negative controlTransfer RNA (tRNA)
      FormLiquid suspension
      FormulationIn PBS.
      Preservative≤0.1% sodium azide
      CommentsAlso recognizes 7-methylguanosine-capped mRNA. 2,2,7-trimethylguanosine (Ab-1) is human, mouse, and rat reactive. This antibody reacts with the exposed 5' cap structure of the snRNAs and the antibody can be used for the purification of snRNPs by immunoaffinity chromatography. All of the major snRNPs bind to the monoclonal antibody column and can be gently eluted by washing with 7-methyl guanosine. Antibody should be titrated for optimal results in individual sample types.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesCasciola-Rosen, L.A., et al. 1994. J. Biol. Chem. 269, 30757-30760.
      Krainer, A.R. 1988. Nuc. Acids Res. 16, 9415.
      Luhrmann, R. 1988. Birntiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 71.
      Reddy, R. and Busch, H., 1988. Birnstiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 1.
      Krainer, A.R. and Maniatis, T. 1985. Cell 42, 725.
      Kramer, A., et al. 1984. Cell 38, 299.
      Lerner, M.R., et al. 1980. Nature 283, 220.
      Application referencesAffinity Purification Luhrmann, R. 1988. Birntiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 71. Immunoprecipitation Moteki, S. and Price, D. 2002. Mol. Cell 10, 599.

      Produits & Applications associés

      Related Products

      Référence Description  
      NA02 Anti-2,2,7-Trimethylguanosine Mouse mAb (K121) Prix & Disponibilité

      Catégories

      Life Science Research > Antibodies and Assays > Primary Antibodies