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PF032 Annexin V-FITC Apoptosis Detection Kit

Annexin V-FITC Apoptosis Detection Kit : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
PF032
  
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      Aperçu

      Tableau de caractéristiques principal

      Detection Methods
      Fluorometric
      Description
      Overview

      This product has been discontinued.



      Optimized for flow cytometry and fluorescence microscopy applications. A RAPID™ protocol has been developed for Annexin V-FITC binding directly in tissue culture media. This obviates the need for tedious centrifugation and wash steps, that increase the occurrence of mechanical membrane disruption. In addition, since apoptosis is a dynamic process that is ongoing once cells are removed from culture conditions and continues throughout experimental processing, the RAPID™ protocol is recommended for the detection of cells in early apoptosis.
      Catalogue NumberPF032
      Brand Family Calbiochem®
      Materials Required but Not Delivered• PBS (137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO4-7H2O, 1.4 mM KH2PO4, adjust pH to 7.4)
      • 2-20 µl, 20-200 µl, and 200-1000 µl precision pipetters with disposable tips
      • Microcentrifuge tubes
      • Adjustable speed microcentrifuge
      • Glass microscope slides (for microscope analysis only)
      • Glass coverslips (for microscope analysis only)
      • dH2O
      • Ice
      • Fluorescence microscope or flow cytometer
      References
      ReferencesFrey, T. 1997. Cytometry 28, 253.
      Darzynkiewicz. Z., et al. 1997. Cytometry 27, 1.
      Boersma, A.W.M., et al. 1996. Cytometry 24, 123.
      Wyllie, A. H. 1993. Br. J. Cancer 67, 205.
      Darzynkiewicz, Z., et al. 1992. Cytometry 13, 795.
      Fadok, V.A., et al. 1992. J. Immunology 148, 2207.
      Kerr, J.F.R., et al. 1972. Cancer 26, 239.
      Product Information
      Detection methodFluorometric
      DeclarationSold under license of U.S. Patent 5,834,196.
      Form100 Tests
      FormatFlow cytometry or fluorescence microscopy
      Kit containsAnnexin V-FITC, 5X Binding Buffer, Propidium Iodide, RAPID™ Media Binding Reagent, and a user protocol.
      Positive controlApoptotic Jurkat cells (dexamethasone or anti-Fas treated)
      Quality LevelMQ100
      Applications
      Key Applications Flow Cytometry
      Immunofluorescence
      Biological Information
      Assay time15 - 30 min
      Sample TypeIntact cells
      Species Reactivity
      • A Broad Range Of Species
      Safety Information
      R PhraseR: 36

      Irritating to eyes.
      S PhraseS: 22-24

      Do not breathe dust.
      Avoid contact with skin.
      Product Usage Statements
      Intended useThe Calbiochem® Annexin V-FITC Apoptosis Detection Kit is a non-isotopic system for the identification of cell membrane alterations that accompany programmed cell death. Detection may be performed by flow cytometry or by fluorescence microscopy.
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage +2°C to +8°C
      Storage ConditionsAnnexin V-FITC kit components are shipped on gel ice pack. Upon receipt, store kit at 4°C. To avoid reagent loss in tube caps, briefly pulse spin all small tubes before removing caps. See Precautions and Recommendations.
      Do not freeze Yes
      Supplemental Information
      Kit containsAnnexin V-FITC, 5X Binding Buffer, Propidium Iodide, RAPID™ Media Binding Reagent, and a user protocol.
      Global Trade Item Number
      Référence GTIN
      PF032 0