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L1613-BC 1 M HEPES Buffer (50x)

A 1 M solution of HEPES that provides good buffering in the pH range of 7.2 to 7.6.

L1613-BC
  
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      Aperçu

      Description
      OverviewHEPES (hydroxyethyl-piperazineethane-sulfonic acid buffer) is an organic buffer and very stable. In the physiological area HEPES has an excellent buffer capacity in a pH range of 7.2 to 7.6. HEPES is apt for all cell culture systems with a high proliferation and cell density. On HEPES buffered cell culture media, the incubation in a CO2 incubator is not necessary.

      Specifications:

      Sterility: Pass
      pH: 7.35-7.55
      Osmolality: 1.65-2.00 Osm/kg H20
      Particles/ml (>10µM): < 25/ml
      Particles/ml (>25µM): < 3/ml
      Color: Colorless
      Appearance: Clear

      How to use HEPES buffer:

      • dissolve powder and adjust pH at 7.2 with sodium hydroxide
      • add 10-20 ml of HEPES buffer per litre of medium (for very delicate cell cultures, use only 10 ml)
      • a concentration of 20 mM HEPES requires 20 ml/l medium, for 25 mM use 25 ml/l
      • if NaHCO3 is required, only add 10%
      Catalogue NumberL1613-BC
      Brand Family Calbiochem®
      Product Information
      Quality LevelMQ300
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Storage +2°C to +25°C
      Do not freeze Ok to freeze
      Global Trade Item Number
      Référence GTIN
      L1613-BC 0