Separation of Rhodamine B in chili extract

Column	Purospher® STAR RP-18 endcapped (5μm) LiChroCART® 250-4,6 HPLC column (150252)
Precolumn	Purospher® STAR RP-18 endcapped (5μm) LiChroCART® 4-4 (150250)
Oven temperature	40 °C
Injection volume	10 μL
Flow Rate	1 mL /min
Eluent A	Phosphoric acid (1 mL 85% Phosphoric acid in to 1 L of Water)
Eluent B	Acetonitrile
Gradient	Time (min)	Eluent A	Eluent B	Flow Rate (mL/min)
	0.0 0.0 15.0 20.0 21.0 35.0	58 58 20 20 58 58	42 42 80 80 42 42	1 1 1 1 1 1
Detection	Fluorescence
Wavelength	Wavelength
PMT Voltage	Medium
Sample Rate	400 ms
Time Constant	1 sec
Sample	0.6248 g Oleoresin oil in 5 mL eluent (Oleoresin sample contaminated with 140 ppb Rhodamine B)