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71731 pIEx/Bac™-3 3C/LIC Vector Kit - Novagen

Novagen's pIEx/Bac vectors are dual-purpose vectors that offer unique flexibility for cloning and expression of proteins in Spodoptera-derived insect cells.

pIEx/Bac™-3 3C/LIC Vector Kit - Novagen: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
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      Descripción

      Replacement Information
      Description
      Overview

      This product has been discontinued.



      The pIEx/Bac™-3 3C/LIC vector is a 3C/LIC prepared version of pIEx/Bac-3. The pIEx/Bac™ vectors are dual-purpose vectors that offer unique flexibility for cloning and expression of proteins in Spodoptera-derived insect cells. These vectors contain the Autographa californica nuclear polyhedrosis virus (AcNPV) enhancer/immediate early promoter combination (hr5/ie1) for plasmid-mediated and early baculovirus expression, and the AcNPV p10 very late promoter for robust baculovirus-mediated expression. All pIEx/Bac plasmids carry AcNPV-derived virus regions to mediate recombination at the polh locus. The pIEx/Bac-3 vector contains an N-terminal His•Tag® coding sequence with 10 histidines followed by an HRV 3C protease cleavage site and a multiple cloning site, with the option to include a C-terminal thrombin protease cleavage site and 8 aa Strep•Tag® II coding sequence.
      Catalogue Number71731
      Brand Family Novagen®
      References
      Product Information
      •20 µg (40 µl) 0.5 µg/µl pIEx/Bac-3 3C/LIC DNA
      •50 µlT4 DNA Polymerase Buffer
      •5 µg (10 µl) 0.5 µg/µl pIEx/Bac 3C/LIC Positive Expression Control
      •25 UT4 DNA Polymerase, LIC-qualified
      •100 µl100 mM DTT
      •40 µl25 mM dATP
      •50 µl25 mM EDTA
      •1.5 ml Nuclease-free Water
      •1 pkgNovaBlue GigaSingles Competent Cells
      •2 mlSOC Medium
      •10 µl Test Plasmid
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage ≤ -70°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      71731 0