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Nuestra amplia cartera de productos consta de paneles multiplex que le permiten elegir, dentro del panel, los analitos que mejor se ajustan a sus requisitos. En una pestaña distinta puede elegir el formato de citocina premezclada o un kit single plex.
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No deben combinarse los siguientes MAPmates™: -MAPmates™ que requieren un tampón de ensayo diferente. -Pares MAPmate™ fosfoespecíficos y totales, por ejemplo, GSK3β y GSK3β (Ser 9). -MAPmates™ con panTyr y específicos de sitio; por ejemplo, receptor del fosfo-EGF y fosfo-STAT1 (Tyr701). -Más de 1 fosfo-MAPmate™ para una sola diana (Akt, STAT3). -La GAPDH y la β-tubulina no pueden combinarse con kits o MAPmates™ que contengan panTyr.
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96-Well Plate
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Opción para ahorrar espacio Los clientes que adquieran múltiples kits pueden optar por ahorrar espacio de almacenamiento retirando el embalaje del kit y recibiendo los componentes de sus ensayos multiplex en bolsas de plástico para un almacenamiento más compacto.
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pET Expression System 9: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
pET Expression Systems and pET pET Expression Systems and pET Expression Systems plus Competent Cells provide core reagents needed for target gene cloning and expression.
Components: pET Expression Systems Components for pET Expression Systems are similar for all systems unless otherwise stated with the specific pET Expression System description. pET Expression Systems include:
• 10 µg pET vector DNA (for each indicated plasmid)
• 0.2 ml BL21 Glycerol Stock
• 0.2 ml BL21(DE3) Glycerol Stock
• 0.2 ml BL21(DE3)pLysS Glycerol Stock
• 0.2 ml Induction Control Glycerol Stock
Components: pET Expression Systems plus Competent Cells
pET Expression Systems plus Competent Cells contain all of the components of the specific pET Expression System, as well as the following additional components, unless otherwise stated with the specific pET Expression System description:
• 0.2 ml NovaBlue Competent Cells
• 0.2 ml BL21(DE3) Competent Cells
• 0.2 ml BL21(DE3)pLysS Competent Cells
• 2 × 0.2 ml SOC Medium
• 10 µl Test Plasmid
These components are sufficient for up to 10 transformations in each host.
Purification and Detection Reagents Purification and detection reagents are
available separately. For complete product descriptions and ordering information, refer
to the Protein Purification and Antibodies, Conjugates & Detection Tools chapters.
pET Expression System 9
The pET Expression System 9 contains 10 µg each of the four versions of pET-9 (pET-9a–d). The pET-9a–d(+) vectors carry an N-terminal T7•Tag® sequence and BamH I cloning site. These vectors are the precursors to many pET family vectors. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map, TB040VM. The pET-3, 9, and 11 vector series are original, basic pET plasmids constructed by Studier and colleagues (Studier 1990) and are the precursors of the subsequent pET vectors. These vectors offer a single BamH I cloning site in three reading frames for producing proteins fused with a non-cleavable Nterminal T7•Tag® epitope. Unfused proteins can be produced by using the Nde I cloning site in the "a", "b", and "c" versions, or the Nco I site in the "d" version. The pET-17b vector carries a multiple cloning site in oneframe.
Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
Catalogue Number
69415
Brand Family
Novagen®
References
References
Studier, F.W., et al. 1990. Meth. Enzymol.185, 60. Seed, B. 1987. Nature329, 840.
Product Information
•
10 µg each
pET-9a-d vector DNA
•
0.2 ml
Host bacterial strains BL21, BL21(DE3), and BL21(DE3)pLysS, glycerol stocks
•
0.2 ml
Induction control clone, glycerol stock
Fusion tag
T7•Tag
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Shipped with Blue Ice or with Dry Ice
Toxicity
Standard Handling
Storage
≤ -70°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia
GTIN
69415
0
Documentation
pET Expression System 9 Certificados de análisis
Cargo
Número de lote
69415
Referencias bibliográficas
Visión general referencias
Studier, F.W., et al. 1990. Meth. Enzymol.185, 60. Seed, B. 1987. Nature329, 840.
JuHyun Kim, et al. (2005) Adenylate kinase of Escherichia coli, a component of the phage T4 dNTP synthetase complex. Journal of Biological Chemistry280, 28221-28229.
Rongkun Shen, et al. (2004) Escherichia coli nucleoside diphosphate kinase interactions with T4 phage proteins of deoxyribonucleotide synthesis and possible regulatory functions. Journal of Biological Chemistry279, 32225-32232.
Ken-Shwo Dai and Choong-Chin Liew. (2001) A novel human striated muscle RING zinc finger protein, SMRZ, interacts with SMT3b via Its RING domain. Journal of Biological Chemistry276, 23992-23999.
Maria D. Koffa, et al. (2001) Herpes simplex virus ICP27 protein provides viral mRNAs with access to the cellular mRNA export pathway. European Molecular Biology Organization Journal20, 5769-5778.
Brian J. Hoffman, et al. (1995) Lactose fed-batch overexpression of recombinant metalloproteins in Escherichia coli BL21(DE3): process control yielding high levels of metal-incorportaed, soluble protein. Protein Expression and Purification6, 646-654.
