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70789 pET Dsb Fusion System 39b

Vectors for export and periplasmic folding of target proteins

pET Dsb Fusion System 39b: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
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      Descripción

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      The pET Dsb Fusion Systems 39b and 40b are designed for cloning and high-level expression of peptide sequences fused with the 208-aa DsbA•Tag™ sequence [pET-39b(+)] or the 236-aa DsbC•Tag™ sequence [pET-40b(+)].

      DsbA and DsbC are periplasmic enzymes that catalyze the formation and isomerization of disulfide bonds, respectively (Rietsch 1996, Sone 1997, Missiakas 1994, Zapun 195, Raina 1997). In contrast to the pET-32 Trx•Tag™ series, which is designed to increase solubility in the cytoplasm, the DsbA•Tag and DsbC•Tag vectors enable potential periplasmic localization and thus may enhance solubility and proper folding in this non-reducing environment (Collins-Racie 1995).








      Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
      Catalogue Number70789
      Brand Family Novagen®
      References
      References

      Rietsch, A., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 13048. Sone, M., et al. 1997. J. Biol. Chem. 272, 10349. Missiakas, D., et al. 1994. EMBO J. 13, 2013. Zapun, A., et al. 1995. Biochemistry 34, 5075. Raina, S. and Missiakas, D. 1997. Ann. Rev. Microbiol. 51, 179. Collins-Racie, L.A., et al. 1995. Bio/Technology 13, 982.
      Product Information
      Components
      Fusion tagHis•Tag, S•Tag
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      70789 0

      Documentation

      pET Dsb Fusion System 39b Certificados de análisis

      CargoNúmero de lote
      70789

      Referencias bibliográficas

      Visión general referencias

      Rietsch, A., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 13048. Sone, M., et al. 1997. J. Biol. Chem. 272, 10349. Missiakas, D., et al. 1994. EMBO J. 13, 2013. Zapun, A., et al. 1995. Biochemistry 34, 5075. Raina, S. and Missiakas, D. 1997. Ann. Rev. Microbiol. 51, 179. Collins-Racie, L.A., et al. 1995. Bio/Technology 13, 982.

      Citas

      Título
    • Paula J. Fernandes, et al. (2007) The type IV Pilin of Burkholderia mallei is highly immunogenic but fails to protect against lethal aerosol challenge in a murine model. Infection and Immunity 75, 3027-3032.
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      Protocolos de usuario

      Cargo
      TB053 Academic and Non-profit Laboratory Assurance Letter
      TB055 pET System Manual

      Mapa vectorial

      Cargo
      TB230 pET-39b Vector Map

      Productos y aplicaciones relacionados

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      Número de referencia Descripción  
      70090 pET-39b(+) DNA - Novagen Precios y disponibilidad
      70091 pET-40b(+) DNA - Novagen Precios y disponibilidad
      70791 pET Dsb Fusion System 40b Precios y disponibilidad

      Productos relacionados por: Application Facete

      Protein Expression

      Categorías

      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors