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70225 pBACgus-6 DNA

pBACgus-6 DNA: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
70225
  
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      Replacement Information
      Description
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      This product has been discontinued.





      pBACgus-6 is a baculovirus transfer plasmid compatible with BacVector™-1000, -2000, or -3000 Triple Cut Virus DNA for low background transfection. A modified gp64 tandem promoter contains both an immediate early promoter for expression beginning immediately after infection and a late promoter for continued expression in the late phase of infection. The pBACgus-6 Transfer Plasmid enables isolation of stably-transfected insect cell lines expressing target proteins. A 20 amino acid gp64 signal peptide sequence is capable of directing high levels of protein into the secretory pathway of infected insect cells. Cloning sites are provided for the creation of N-terminal fusions of an insert with His•Tag® and/or S•Tag™ sequences. Unique restriction sites are indicated on the circle map. The cloning/expression region of the coding strand transcribed from the gp64 promoter is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand. Single stranded sequencing of phage-derived DNA can be performed using the 1629DWN primer.
      Catalogue Number70225
      Brand Family Novagen®
      References
      Product Information
      Applications
      Biological Information
      Physicochemical Information
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      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      70225 0

      Documentation

      pBACgus-6 DNA Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      pBACgus-6 DNA Certificados de análisis

      CargoNúmero de lote
      70225

      Folleto

      Cargo
      Insect Cell Expression

      Citas

      Título
    • Naoto Yonezawa, et al. (2005) Recombinant porcine zona pellucida glycoproteins expressed in Sf9 cells bind to bovine sperm but not to porcine sperm. Journal of Biological Chemistry 280, 20189-20196.
      		•

      Mapa vectorial

      Cargo
      TB222 pBACgus-6 Vector Map

      Secuencia vectorial

      Cargo
      pBACgus-6 Sequence

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      Categorías

      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Insect Expression > Insect Expression Vectors & Kits