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No deben combinarse los siguientes MAPmates™: -MAPmates™ que requieren un tampón de ensayo diferente. -Pares MAPmate™ fosfoespecíficos y totales, por ejemplo, GSK3β y GSK3β (Ser 9). -MAPmates™ con panTyr y específicos de sitio; por ejemplo, receptor del fosfo-EGF y fosfo-STAT1 (Tyr701). -Más de 1 fosfo-MAPmate™ para una sola diana (Akt, STAT3). -La GAPDH y la β-tubulina no pueden combinarse con kits o MAPmates™ que contengan panTyr.
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Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
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96-Well Plate
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Añadir más reactivos (Se necesita tampón y un kit de detección para usar con MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Opción para ahorrar espacio Los clientes que adquieran múltiples kits pueden optar por ahorrar espacio de almacenamiento retirando el embalaje del kit y recibiendo los componentes de sus ensayos multiplex en bolsas de plástico para un almacenamiento más compacto.
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Bead-based flow cytometric xMAP® assays enable sensitive, precise detection of analytes within a sample. When directed toward proteins, such assays are essentially sandwich immunoassays on a bead. Samples are combined with fluorescently labeled microparticles (beads) covalently conjugated to a capture antibody. Analytes captured on the beads are identified with detection antibodies and a fluorescent label. A major hallmark of bead-based assays over traditional protein detection methods (such as ELISA) is the capacity for multiplexing, as bead-based assays allow simultaneous detection of multiple analytes in a small sample volume. Other advantages of xMAP® assays include flexibility, robustness, simple sample handling, and requirement of minimal sample volumes, making them an ideal platform for immunodetection of biomarkers and signaling proteins.
Receptor tyrosine kinases (RTKs) are critical regulators of numerous cell signaling pathways and have been implicated in various disease states. Ligand binding to the extracellular domain of transmembrane RTKs triggers receptor dimerization and autophosphorylation of an intracellular kinase domain. This event ultimately triggers activation of downstream pathway proteins via phosphotyrosine-SH2 domain interactions. The WideScreen® RTK Assay Kits provides users with the ability to quantify the following RTKs, in either its phosphorylated or total state: • Epidermal Growth Factor Receptor (EGFR) • Hepatocyte Growth Factor Receptor (HGFR) • Human Epidermal Growth Factor Receptor 2 (HER-2) • Insulin-Like Growth Factor 1 Receptor (IGF-1R) • Platelet-Derived Growth Factor Receptor beta (PDGFRβ) • Tyosine Kinase with Immunoglobulin and EGF Repeats 2 (Tie-2) • Total Vascular Endothelial Growth Factor Receptor 2 )
If expressed in the cell line of interest, individual RTK proteins may be present in either inactive or active states depending on availability of the RTK ligand. In the WideScreen® assay, cellular protein samples are prepared by using a Cell Extraction Kit and WideScreen® Reagent Kit, both of which can be purchased separately or are already included in the WideScreen® RTK Total Assay Complete Kit and the WideScreen® RTK pTyr Assay Complete Kit. Extracts are combined with dye-labeled Luminex® xMAP® beads covalently conjugated to capture antibodies specific to each phosphorylation site. After incubation and several washes, the beads are incubated with biotin-labeled detection antibodies. The resulting bead-immobilized immunosandwich is detected with streptavidin-phycoerythrin and acquired using a Luminex® xMAP® instrument.
Importantly, detection antibodies differ between the WideScreen™ Total RTK assay and the WideScreen™ pTyr RTK assay. In the Total assay, the detection antibody recognizes a second epitope on the RTK, allowing quantification of RTK levels without regard to phosphorylation state. In the pTyr assay, the detection antibody is specific to pTyr on all RTKs. Relative quantification of RTK phosphorylation (expressed in median fluorescence intensity units) is possible using the pTyr kit. Because antibodies would compete for the same analyte, the RTK Total Assays cannot be multiplexed with the RTK pTyr assays.
The WideScreen® Total Assay Complete Kit and the WideScreen® RTK pTyr Assay Complete Kit comprises the entire set of reagents to run 96 test wells for EGFR, HGFR, HER-2, IGF-1R, PDGFRβ, Tie-2, and VEGFR2, including the RTK pTyr 7-plex in either its phosphorylated or total state, a Cell Extraction Kit, and a WideScreen® Reagent Kit. Standards are included in the WideScreen® Total Assay Complete Kit, but not the WideScreen® pTyr Assay Complete Kit
The RTK recombinant standards are used to create standard curves when performing quantitative assays. For a table of standard concentrations, please refer to TB500 read the appendices carefully. Each recombinant standards mix contains reagents sufficient to generate eight singleplex or multiplex standard curves, or four standard curves in duplicate.
Catalogue Number
71943
Brand Family
Novagen®
Safety Information
R Phrase
R: 20/21-25-36/37/38-41
Harmful by inhalation and in contact with skin. Toxic if swallowed. Irritating to eyes, respiratory system and skin. Risk of serious damage to eyes.
S Phrase
S: 23-24/25-26-36/37/39-A09
Do not breathe fumes. Avoid contact with skin and eyes. In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing, gloves and eye/face protection.